TEST DIRECTORY

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Laboratory:Akiruno

Simultaneous identification of Neisseria gonorrhoeae and Chlamydia trachomatis rRNA(Suspended beyond orders placed 09-02-2021)

  • TEST NAME SPECIMEN
    REQUIREMENT
    (mL)
    CONTAINER CAP COLOR STORE
    TEMPERATURE
    (STABILITY)
    TURNAROUND
    TIME (DAY)
    METHODOLOGY REFERENCE RANGE
    (UNIT)
  • INACTIVE
    Simultaneous identification of Neisseria gonorrhoeae and Chlamydia trachomatis rRNA(Suspended beyond orders placed 09-02-2021)
    Partial urine
    2.0
    V20 Refrigeration
    (30 days)
    2-4 TMA

    TMA (Transcription mediated amplification)
    A method of amplifying RNA using two types of enzymes and two types of primers and substrates.
    Double-stranded DNA is synthesized from the extracted RNA using reverse transcriptase, and this double-stranded DNA is used as a template to synthesize RNA by RNA polymerase transcription reaction, which is repeated to amplify the desired RNA region.

    Negative

TEST NAME

SPECIMEN
REQUIREMENT
(mL)

CONTAINER

INACTIVE
Simultaneous identification of Neisseria gonorrhoeae and Chlamydia trachomatis rRNA(Suspended beyond orders placed 09-02-2021)

Partial urine
2.0

CAP COLOR

STORE
TEMPERATURE
(STABILITY)

TURNAROUND
TIME (DAY)

Refrigeration
(30 days)

2-4

METHODOLOGY

REFERENCE RANGE
(UNIT)

TMA

TMA (Transcription mediated amplification)
A method of amplifying RNA using two types of enzymes and two types of primers and substrates.
Double-stranded DNA is synthesized from the extracted RNA using reverse transcriptase, and this double-stranded DNA is used as a template to synthesize RNA by RNA polymerase transcription reaction, which is repeated to amplify the desired RNA region.

Negative

COMMENT


Avoid freezing. Please avoid duplicate requests of other items. Please transfer the specimen to the special container and submit as soon as possible after collection. Please adhere strictly to the collection method or it may become impossible to report test results, especially for secretions, which may increase in the viscosity of the specimen. Since the affects of contamination are greater in this test method, please collect and handle the specimen with care.

CONTAINER

V20  Previous container symbol F2

(for urine / mouth washing liquid)

(for urine / mouth washing liquid)

Contents: Lithium lauryl-sulfate, additives
Storage method: Room temperature
Expiration period: 1 year from production

(for secretion)

(for secretion)

Contents: Lithium lauryl-sulfate, additives
Storage method: Room temperature
Expiration period: 1 year from production

supplementary information

V20 (Previous container symbol F2) handling methods of samples

Obtaining specimens from urine

  1. Urinate at least 1 hour before collecting sample.
  2. Collect 20 to 30 mL of the first-voided urine in a cup. (Please note that too much urine will cause dilution of the specimen, reducing the detection sensitivity.)
  3. Within 24 hours, pipette 2 mL of urine in the dedicated container using an attached dropping pipette, and mix. If the urine is filled to the level between the black lines on the dedicated container, an appropriate amount of urine is collected.
  4. After the sampling, submit the specimen under refrigerated conditions.

Obtaining specimens from the cervical canal

  1. Remove excess mucus from the cervical canal and the surroundings using the cleaning swab (white one). Discard the cleaning swab.
  2. Insert the collecting swab (blue one) into the cervical canal.
  3. Gently rotate the swab clockwise for 10 to 30 seconds to ensure adequate sampling (Figure 1).
  4. Withdraw the swab carefully while avoiding any contact with the vaginal mucus.
  5. Immediately place the swab into the dedicated container, and mix the specimen with the preservation solution in the container (Figure 4).
  6. Carefully break the swab shaft at the score line (Figure 5). Do not spill the contents of the tube.
  7. Close the cap firmly while the swab is included in the dedicated container. Submit the specimen under refrigerated conditions (Figure 5).

Obtaining specimens from the urethra of men

  1. Urinate at least 1 hour before collecting sample.
  2. Insert the collecting swab (blue one) 2 to 4 cm into the urethra (Figure 2).
  3. Gently rotate the swab clockwise for 2 to 3 seconds to ensure adequate sampling (Figure 2).
  4. Immediately place the swab into the dedicated container, and mix the specimen with the preservation solution in the container (Figure 4).
  5. Carefully break the swab shaft at the score line (Figure 5). Do not spill the contents of the tube.
  6. Close the cap firmly while the swab is included in the dedicated container. Submit the specimen under refrigerated conditions (Figure 5).

Obtaining specimens from the phyarinx (throat swab specimen)

  1. Rub the collecting swab (blue one) firmly over the tonsillar arches to ensure adequate sampling (Figure 3).
    Before obtaining a throat swab specimen, patient must refrain from washing month, eating, or drinking.
  2. Immediately place the blue swab into the transport tube, and mix the specimen with the swab transport medium.
  3. Carefully break the blue swab shaft at the score line (Figure 4). Do not spill the contents of the tube.
  4. Firmly tighten the cap of the swab transport tube, and submit the specimen under refrigerated conditions (Figure 5).

Obtaining mouth wash specimens

  1. Patient must refrain from eating, gargling, brushing teeth, or chewing gum before collecting specimens.
  2. Have the patient to sit face to face to the examiner.
  3. Take 15 to 20 mL of physiological saline (0.9% saline solution) in the month, tilt the head backward, and gargle vigorously for 15 to 20 seconds.
  4. Collect all of the gargling liquid into the container for mouth wash specimen.
  5. Within 24 hours, pipette 2 mL of the mouth wash specimen in the dedicated container using an attached dropping pipette. If the specimen is filled to the level between the black lines on the dedicated container, an appropriate amount is collected.
  6. After the sampling, submit the specimen under refrigerated conditions.

[Note]
Please note that the swab shaft may be broken if an excessive force is applied to the shaft while collecting sample.

figure 1 figure 2 figure 3 figure 4 figure 5

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