Laboratory:Akiruno

Laboratory:Akiruno
CODE:06130 0
FISH (Fluorescence in situ hybridization)
Fluorescence in situ hybridization
Hybridizes with the target DNA using a probe labeled with a fluorescent dye, and uses the fluorescent site that develops at a specific wavelength as a signal on the chromosome. How to detect under fluorescence microscope.
There is a direct method in which a probe labeled with a fluorescent dye is directly bound to the target DNA, and an indirect method in which the probe labeled with a labeling substance is bound to the target DNA, and then the labeled probe and fluorescent substance are bound to develop color. .
Please use slides coated with silane, etc., and after slicing, dry overnight at approximately 40℃ before submitting promptly. Even when baking to prevent tissue detachment, do it as quickly as possible (within 1 hour) and avoid leaving the glass slide at high temperatures for longer than that. The test guide created by the Gastric Cancer HER2 Test Pathology Subcommittee states that the fixed material should be fixed in 10% neutral buffered formalin for 6 to 72 hours (in the case of biopsy specimens, according to the size of the specimen; however, the fixation time is 6 hours or more). considered desirable. Slice the tissue into 4-6 μm thick slices.
[オブジェクトケース]
プレパラート (スライドグラス)
貯蔵方法:室温