TEST DIRECTORY

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Laboratory：Akiruno

○Epithelial cell membrane antigen (EMA)

CODE：05923 1

  • TEST NAME SPECIMEN SUBMISSION SPECIMEN
    REQUIREMENT
     CONTAINER CAP COLOR STORE
    TEMPERATURE
    (STABILITY)     TURNAROUND
    TIME (DAY)     METHODOLOGY
  • Epithelial cell membrane antigen (EMA)
    Formalin-fixed tissue (paraffin block/unstained specimen slides are also available) VP0 Room temperature
     *5-8

COMMENT


*Required number of days
・If degreasing and deashing are required, the required number of days will be 7 to 12 days.
　・If enzyme antibody staining is required, it will take approximately 2 weeks.
・If fluorescent antibodies are required, the required time will be 2 to 3 weeks.
　・If a bone marrow image is required, it will take approximately 2 weeks.
　・Organs that require diagnosis by a specialist (skin, liver, bone marrow, heart, etc.) require 7 to 12 days.
　・Surgical materials (when cutting out more than 5 blocks) will take 2 to 3 weeks.
　・ In the case of special requests, if there is a large number of requests (approximately 20 or more depending on the processing content), the required number of days will be longer than usual, so please contact our sales staff in advance.
About the pathology container (VP0)
Please fill the pathology container (VP0) with 20% formalin solution, fix the tissue, and submit.
If the tissue is too large to fit into the pathology container (VP0), please fix it thoroughly and submit it in a Histopack (extra large, large, medium, small).

CONTAINER

supplementary information

List of enzyme antibody staining

Room temperature [Materials: formalin-fixed tissues (Paraffin blocks or unstained slides are also available.)]
CODE TEST NAME ANTIGEN DISTRIBUTION CLINICAL SIGNIFICANCE
TUMOR-RELATED
5901 0
 CEA Normal colonic mucosa, fetal gastric mucosa (pyloric region), ducts of the salivary gland from fetuses Many adenocarcinomas, some squamous cell carcinomas, some carcinoids, medullary carcinoma of thyroid, some small cell lung cancers
5902 7
 AFP (α-fetoprotein) Hepatocytes/yolk sac/intestinal epithelium of the fetal period Hepatocellular carcinoma, yolk sac tumor, some stomach cancers, immature teratoma, pancreatoblastoma
5906 6
 NSE Nerve cells, nerve fiber, (some) endocrine cells, adrenomedullary cells Neuroendocrine tumor, melanoma, myogenic tumor
5907 3
 HCG Placental villous syncytiotrophoblast Choriocarcinoma, some adenocarcinomas including stomach cancer, carcinoid (α-subunit), germinoma, large cell lung cancer, malignant islet-cell carcinoma
IMMUNOGLOBULINS AND COMPLEMENT
5908 1
 IgG B lymphocytes, plasma cells Plasmacytoma, myeloma, primary glomerular disease, glomerular disease associated with other diseases
5909 8
 IgA
5910 8
 IgM
5911 5
 κ chain Plasmacytoma
5912 2
 λ chain
5913 0
 C1q Primary glomerular disease, glomerular disease associated with other diseases
5914 7
 C3
5916 1
 Fibrinogen
TISSUE MARKERS
5923 5
 Epithelial membrane antigen (EMA) Many glandular epithelia/ductular epithelia, some mesothelia Many adenocarcinomas, squamous cell carcinoma, (some) mesotheliomas, synovial sarcoma, chordoma
5925 0
 Keratin (wide range) Almost all epithelial cells, mesothelial cells Squamous cell carcinoma, some adenocarcinomas, mesothelioma, adenomatoid tumor, small cell lung cancer, medullary carcinoma of thyroid, carcinoid, epithelioid sarcoma, synovial sarcoma, chordoma, thymoma, choroid plexus papilloma
5926 7
 High molecular keratin (34βE12) Squamous cells Squamous cell carcinoma
5928 1
 Vimentin Mesenchymal cells, epithelial cells in the mitotic phase, tubular epithelium of fetal kidney Mesenchymal tumor, some cancers (e.g., Grawitz tumor, thyroid cancer), (some) undifferentiated carcinomas
5929 9
 Myoglobin Skeletal muscle, thymic medullary myoid cells Rhabdomyosarcoma, tumor components indicating differentiation to striated muscle
5930 9
 Desmin Smooth muscle cells, skeletal muscle cells Muscular tumor
5931 6
 S100 protein Astrocytes, Schwann cells, satelite cells (neuromuscular cells), chondrocytes, fat cells, T-zone histiocytes, Langerhans cells (skin), myoepithelial cells Glioma, schwannoma, nevus, melanoma, paraganglioma (satelite cells), (some) fibrous histiocytomas, histiocytosis X, chondrosarcoma, chordoma, liposarcoma, mixed tumor of salivary gland
5932 3
 Glial fibrillary acidic protein (GFAP) Astrocytes, ependymal cells Glioma, ependymoma, medulloblastoma, choroid plexus papilloma
5933 1
 Neurofilament (NF) Nerve cells, nerve fiber, adrenomedullary cells, (some) endocrine cells Neuroblastoma, pheochromocytoma, carcinoid, small cell lung cancer, medullary carcinoma of thyroid, pineocytoma
5934 8
 Factor VIII-related antigen Vascular endothelium, (some) lymphatic endothelia, megakaryocytes Angioma, Kaposi sarcoma
5935 5
 Prostate-specific antigen (PA) Prostate epithelium Prostate cancer
5936 2
 Prostatic acid phosphatase (PSAP) Prostate epithelium, some endocrine cells Prostate cancer, some endocrine gland tumors
5937 0
 Lysozyme Paneth cells, pyloric gland, salivary gland, bronchial gland, bile duct accessory gland, tubular epithelium, granulocytes, macrophages Adenocarcinoma derived from the gastric/pulmonary/biliary system, salivary gland tumor, histiocytic tumor
HORMONES (PANCREAS/GUT)
5942 9
 Glucagon A-cells of the Langerhans islets Langerhans adenoma, ectopic hormone-producing tumor
5943 6
 Gastrin G-cells in the stomach/intestines
5944 3
 Somatostatin D-cells of the Langerhans islets, D-cells in the stomach/intestines
5947 5
 Serotonin Normal endocrine cells Carcinoid
HORMONES (PLACENTAL FUNCTION)
5950 0
 Placental alkaline phosphatase (PLAP) Placental villous syncytiotrophoblast, reserve cells of cervical cancer, endometrial lining epithelium Seminoma, some adenocarcinomas, cervical cancer (exceptional)
HORMONES (THYROID GLAND/PARATHYROID FUNCTION)
5886 6
 Calcitonin Thyroid C cells, bronchial K cells Medullary carcinoma of thyroid, small-cell lung cancer, bronchial carcinoid, Langerhans tumor (in some cases)
5887 3
 Parathyroid hormone (PTH) Parathyroid cells Parathyroid cancer, ectopic hormone-producing tumor
HORMONES (PITUITARY FUNCTION)
5890 8
 Thyroid stimulating hormone (TSH) Anterior pituitary gland Pituitary adenoma, ectopic hormone-producing tumor
5891 5
 Growth hormone (GH)
5892 2
 Luteinizing hormone (LH)
5895 4
 Prolactin (PRL)
5896 1
 Antidiuretic hormone (ADH) Posterior pituitary gland
LYMPHOCYTE-RELATED
5917 9
 Leukocyte common antigen (LCA) Lymphocytes, histiocytes (in some cases) Malignant lymphoma, plasmacytoma, chloroma
5918 6
 T lymphocytes (UCHL-1) T lymphocytes, polymorphonuclear leukocyte, histiocytes (in some cases) Malignant T lymphoma
5920 3
 B lymphocytes (L-26) B lymphocytes Malignant B lymphoma
5899 3
 Leu M1 Polymorphonuclear leukocyte, monocytes, activated T lymphocytes, normal epithelial cells, proximal tubular epithelium Hodgkin lymphoma

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