Laboratory:Akiruno
- TOP
- gene-related test
- genetic tests related to drug response
- NUDT15 gene codon 139 polymorphism analysis
Laboratory:Akiruno
○NUDT15 gene codon 139 polymorphism analysis
CODE:05881 8
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TEST NAME
SPECIMEN
REQUIREMENT
(mL) CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT) -
NUDT15 gene codon 139 polymorphism analysis
Blood (EDTA-2Na added)
2.0
PN2,PN5
(3 days)
4-8 PCR (Real Time PCR)Real-time PCR
It is a type of nucleic acid amplification methods based on PCR method as the basic principle, which is a measurement method that, by using oligonucleotides that emit fluorescence when degraded, enables quantitative of target nucleic acids in real time by confirming the fluorescence signals in each PCR cycle.
COMMENT
Avoid freezing. This item detects genetic polymorphisms of the NUDT15 gene codon 139 and reports them in amino acid notation (Arg/Arg, Arg/Cys, Arg/His, Cys/Cys, Cys/His, His/His).
Please avoid duplicate requests of other items.
Since the affects of contamination are greater in this test method, please handle specimens with care when collecting them.
Subject to PGx (see below)
Collect in the container shown below, mix well, and preserve refrigerated.
Please avoid duplicate requests of other items.
Since the affects of contamination are greater in this test method, please collect and handle the specimens with care.
CONTAINER
PN2 旧容器記号 C C3 4
EDTA-2Na入り (真空採血量2mL)
内容:EDTA-2Na 3.0mg
貯蔵方法:室温
有効期間:製造から2年
PN5 旧容器記号 C C3 4
EDTA-2Na入り (真空採血量5mL)
内容:EDTA-2Na 7.5mg
貯蔵方法:室温
有効期間:製造から2年
supplementary information
NUDT15遺伝子codon 139多型解析の検出対象
塩基配列 | アミノ酸 | NUDT15遺伝子多型 |
---|---|---|
CGT | アルギニン (Arg) | 野生型 |
TGT | システイン (Cys) | c.415C > T (rs116855232) |
CAT | ヒスチジン (His) | c.416G > A (rs147390019) |