TEST DIRECTORY

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Laboratory：Akiruno

○Estradiol (E2)（Suspended beyond orders placed 11-27-2019）

CODE：05841 2

  • TEST NAME SPECIMEN
    REQUIREMENT
    （ｍL）     CONTAINER CAP COLOR STORE
    TEMPERATURE
    (STABILITY)     TURNAROUND
    TIME (DAY)     METHODOLOGY REFERENCE RANGE
    (UNIT)
  • INACTIVE
    Estradiol (E2)（Suspended beyond orders placed 11-27-2019）
    Urine storage
    3.0
    U00 Freeze
     8-14 RIA ammonium sulfate salting out method

    RIA (Radio immunoassay)
    Radioimmunoassay
    A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity.
    As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.

     See below

TEST NAME

SPECIMEN
REQUIREMENT
（ｍL）

CONTAINER

INACTIVE
Estradiol (E2)（Suspended beyond orders placed 11-27-2019）

Urine storage
3.0

CAP COLOR

STORE
TEMPERATURE
(STABILITY)

TURNAROUND
TIME (DAY)

Freeze

8-14

METHODOLOGY

REFERENCE RANGE
(UNIT)

RIA ammonium sulfate salting out method

RIA (Radio immunoassay)
Radioimmunoassay
A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity.
As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.

See below

COMMENT


Please specify your sex. If you are pregnant, please indicate the number of weeks of pregnancy.

CONTAINER

Urine containers

U00 Previous container symbol Y

Urine containers

Storage method: Room temperature

supplementary information

Reference ranges of estrogen fractionatio, Urine

[04339 1] 3 fractionation　 [05121 1] E1　 [05841 2] E2　 [05122 0] E3

(Unit：μg/day)
Estrone Estradiol Estriol
Non-pregnant
women		 Female Follicular phase 1.00 – 8.00 0.50 – 5.00 1.00 – 8.00
Ovulatory phase 2.00 – 20.0 2.00 – 10.0 2.00 – 20.0
Luteal phase 5.00 – 20.0 5.00 – 20.0 5.00 – 30.0
Male 0.30 – 10.0 0.10 – 3.00 0.30 – 10.0
Pregnant
women		 Weeks 21 – 24 410 – 2630 369 – 1270 6700 – 23700
Weeks 25 – 28 465 – 3140 368 – 1500 8250 – 31500
Weeks 29 – 32 379 – 3360 582 – 1500 9450 – 33400
Weeks 33 – 36 445 – 3960 561 – 2530 11500 – 74200
Weeks 37 – 40 465 – 5490 683 – 3130 17400 – 87300

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