RAS/BRAF gene mutation analysis

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gene-related test
genetic tests for malignant tumor (solid tumor)
RAS/BRAF gene mutation analysis

Laboratory：Akiruno

○RAS/BRAF gene mutation analysis

CODE：05755 8

TEST NAME SPECIMEN
REQUIREMENT
（ｍL） CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT)
RAS/BRAF gene mutation analysis
Unstained specimen slide
5 sheets
thickness 5 to 10μm
Z10
4-7 PCR-rSSO method

COMMENT

The purpose of this test is to analyze mutations in RAS and BRAF genes (KRAS and NRAS genes are exon2codon12,13, exon3codon59,61, exon4codon117,146, BRAF gene is exon15codon600) in pathology materials. The purpose of this project is to analyze mutations of BRAF gene (exon15codon600). Only pathology materials are accepted.
The percentage of tumor cells required for testing is at least 10%.
See below for notes on submitting unstained specimen slides.
Avoid duplicate requests in one sample.
●Notes
It is a prerequisite that tissue samples used for this test have been histopathologically evaluated and the presence and content of tumor tissue has been confirmed. In the case of a test performed to assist in the selection of treatment for unresectable advanced or recurrent colorectal cancer, the insurance claim names are “BRAF gene test” and “RAS gene test” which are prescribed points for malignant tumor gene test (2 items).
●Submission Conditions
Unstained specimen slides should be histopathologically evaluated to ensure that the required percentage of tumor cells (tumor cells as a percentage of total cells in the specimen) is present. If the required percentage is not met, mark the tumor cell area from the back of the unstained specimen slide. Note that submission of unstained specimen slides without marking may result in failure of macrodissection, which may affect the results, such as a false negative result.
●About unstained specimen slides
Immediately immerse the collected tissue in a 10% neutral buffered formalin solution and fix it (recommended fixation time is 6 to 48 hours). When submitting, please prepare serial sections at the specified thickness from formalin-fixed paraffin-embedded (FFPE) blocks prepared within the last 3 years, if possible. Please be careful to avoid contamination by changing the microtome blade for each specimen when slicing. Please note that as nucleic acids are fragmented due to formalin fixation of tissues, analysis may not be possible depending on the type and composition of the fixative, fixation time, and storage condition of the specimen after fixation.
●About biopsy specimens
Biopsy specimens often contain only a small amount of specimen, and there is a possibility that most of the tissue itself has disappeared or that the tissue has become a piece of tissue that does not contain tumor cells. Be aware of this in advance.

CONTAINER

Z10 旧容器記号 t 30

[オブジェクトケース]
プレパラート (スライドグラス)

貯蔵方法：室温

TEST DIRECTORY