RAS/BRAF gene mutation analysis

TOP
gene-related test
genetic tests for malignant tumor (solid tumor)
RAS/BRAF gene mutation analysis

Laboratory：Akiruno

○RAS/BRAF gene mutation analysis

CODE：05755 8

TEST NAME SPECIMEN
REQUIREMENT
（ｍL） CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT)
RAS/BRAF gene mutation analysis
Unstained specimen slide
5 pieces 5-10μm
Z10
4-7 PCR-rSSO method

COMMENT

We aim to analyze mutations in the RAS and BRAF genes (codons 12 and 13 of exon 2, codons 59 and 61 of exon 3, codons 117 and 146 of exon 4 for both KRAS and NRAS genes, codon 600 of exon 15 for BRAF gene) in pathology materials. Only pathology materials can be contracted.
The percentage of tumor cells required for the test is at least 10%. Please refer to the following for notes on submitting unstained specimen slides.
Please avoid duplicate requests of other items.
[5755 9]Notes on RAS and BRAF Gene Mutation Analysis
It is a prerequisite for contracting that the tissue fragments to be provided for this test must have been histopathologically evaluated and that the presence and content of tumor tissue must have been confirmed. The insurance claim names for tests performed to assist in the selection of treatment in unresectable advanced or recurrent colorectal cancer are “BRAF gene test” and “RAS gene test,” which are prescribed points for the malignant tumor gene test (two items).
●Submission Conditions
Unstained specimen slides should have been histopathologically evaluated to ensure that the tumor cells are present in at least the required percentage for the test. If the tumor cell percentage is not satisfied, mark the tumor cell area from the back of the unstained specimen slides. Please note in advance that, if the specimens are submitted without marking, macrodissection cannot be performed, which may affect the results such as a false negative result.
●About Unstained Specimen Slides
The collected tissue should be immediately immersed in a 10% neutral buffered formalin solution for fixation (recommended fixation time: 6-48 hours). When submitting, please prepare serial sections of the designated thickness from formalin-fixed, paraffin-embedded (FFPE) blocks prepared within the last 3 years to the extent possible. Please be careful to avoid contamination by changing the microtome blade for each s

CONTAINER

Z10 旧容器記号 t 30

[オブジェクトケース]
プレパラート (スライドグラス)

貯蔵方法：室温

TEST DIRECTORY