Laboratory：Akiruno
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Laboratory：Akiruno
○Preparation of stained specimens (see below)
CODE：05653 1
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TEST NAME
SPECIMEN SUBMISSION
SPECIMEN
REQUIREMENT
CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY -
Preparation of stained specimens (see below)
Unstained specimen slide 2 unstained slide specimens Z10
*5-7
TEST NAME | SPECIMEN SUBMISSION | SPECIMEN |
|---|---|---|
Preparation of stained specimens (see below)
| Unstained specimen slide | 2 unstained slide specimens |
CONTAINER | CAP COLOR | STORE |
|---|---|---|
|
TURNAROUND | METHODOLOGY |
|---|---|
*5-7 |
|
COMMENT
Please prepare smear specimen, place them in the following object case, and submit them at room temperature.
CONTAINER
![[Object cases] Preparation (slide glass)](../../common/yoki-images/z10.jpg)
Z10 Previous container symbol t
[Object cases] Preparation (slide glass)
Storage method: Room temperature
supplementary information
List of various staining techniques
| STAINING METHOD | PURPOSE | RESULT | COMMENT | ||
|---|---|---|---|---|---|
| Hematoxylin-eosin (HE) staining | General staining | Cell nuclei, cartilage, bacteria, undecalcified and calcified parts: Indigo blue (hematoxylin). Cytoplasm, connective tissues, muscular tissues, red blood cells: Various colors of deep red to red (eosin) |
Basic staining for all staining techniques |
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| Elastica van Gieson staining | Staining of connective tissues | Collagen fiber | Collagen fiber: Red (acid fuchsin). Muscular fiber, red blood cells, cytoplasm: Yellow (picric acid). Nuclei: Blackish brown (Weigert’s iron hematoxylin). Elastic fiber: Purple-black. | The van Gieson staining alone is also used. |
|
| Azan staining | Collagen fiber, reticular fibers, renal glomeruli, renal tubular basement membrane, mucus, cartilage: Blue (aniline blue). Nuclei, cytoplasm, muscular tissues, fibrin: Red to deep red (azocarmine G). Red blood cells: Red to red-orange (azocarmine G, orange G) | Acid fuchsin staining can also be used. |
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| Masson’s trichrome staining | Nuclei: Blackish brown (Weigert’s iron hematoxylin). Depending on stains used, different results are obtained. | Various modified methods are available. |
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| Resorcin-fuchsin staining | Elastic fiber | Elastic fiber: Purple-black | This is a representative technique of staining and also used for double staining with van Gieson staining. |
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| Orcein staining | Elastic fiber, HBs antigen: Dark reddish-brown | This is also used as HBs antigen staining. |
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| Silver staining (silver impregnation) | Reticular fibers | Argyrophilic fibers (reticular fibers): Black Collagen fiber: Red-purple |
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| Phosphotungstic acidhematoxylin (PTAH) staining | Fibrin, glia tissues | Stripes in the striated muscles, fibrin, nuclei: Indigo blue Neuroglia cells, neuroglia fiber: Dark reddish-brown |
Stripes in the striated muscles are stained. |
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| Periodic acid-Schiff (PAS) reaction | Polysaccharide staining | Glycogen | Glycogen: Red to red-purple. Nuclei: Indigo blue (hematoxylin) | Mucus, mast cell granules, amebas, and fungi are also stained. |
|
| Alcian blue staining | Acid mucopolysaccharide | Acid mucopolysaccharide: Blue. Nuclei: Red (Kernechtrot) | This is also used for double staining with PAS. |
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| Mucicarmine staining | Mucus | Mucus: Red. Nuclei: Indigo blue (hematoxylin) | Epithelial mucus is well stained. |
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| Toluidine blue staining | Mucus, mast cell granules, cartilage matrix: Red-purple. Nuclei: Blue | ||||
| Congo red staining | Amyloid staining | Amyloid: Red-orange. Nuclei: Indigo blue (hematoxylin) | Permanganic acid treatment can differentiate amyloid. |
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| Dylon (direct fast scarlet) staining | Amyloid: Orange. Nuclei: Indigo blue (hematoxylin) | ||||
| Grimelius (argyrophil) staining | Differential staining of endocrine cells | Argyrophilic cells in the digestive tract, argyrophilic cells in the pituitary gland, carcinoid, A-cells in the islets of Langerhans in the pancreas, endocrine tumor cells: Blackish brown | Bouin fixation is also available. |
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| Fontana-Masson staining | Argentaffin cells in the digestive tract, argentaffin carcinoid, melanocyte: Blackish brown | This is also used to confirm evidence of melanocytes. |
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| Kossa staining | Staining of inorganic substances in tissues | Calcium | Calcium deposits: Blackish brown | Avoid using a noble metal-containing fixative solution. |
|
| Berlin blue staining | Iron | Hemosiderin: Blue. Background: Pink (Kernechtrot) | Hemosiderin accounts for most part of the substances to be stained. |
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| Rubeanic acid staining | Copper staining | Copper: Black-green | |||
| Loeffler’s methylene blue staining | Staining of pathogenic microorganisms in tissues | Common bacteria | Simple staining | Bacteria: Deep blue. Nuclei, background: Light blue | |
| Hucker-Conn gram staining | Gram staining | Gram-positive bacteria: Deep blue Gram-negative bacteria: Red |
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| Ziehl-Neelsen method of acidfast bacteria staining | Acid-fast bacteria staining | Acid-fast bacteria: Red. Background: Light blue (methylene blue) | |||
| Grocott staining | Fungi | Fungi: Black. Background: Green (light green) | Glycogen and mucus are also stained black. |
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| Orcein staining | HBs antigen | HBs antigen, elastic fiber: Dark reddish-brown | HBs antigen is diffusely stained in intracytoplasmic inclusion bodies or cells. |
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| Victoria blue staining | HBs antigen, elastic fiber: Blue | This is also used for elastic fiber staining. |
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| Luxol fast blue staining (Klüver-Barrera staining) | Staining of the central nervous tissue | Double staining of medullary sheath and Nissl body | Medullary sheath: Blue-green Nissl body, nuclei: Purple |
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| Simple staining of Nissl body | Nissl body: Purple | ||||
| Periodic acid-methenamine silver (PAM) staining | Staining of the kidney | Renal glomerular basement membrane, reticular fibers: Black | This is also used for many purposes as well as for the kidney. |
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| Giemsa staining | Staining of blood cells in tissues | Nuclei: red-purple. Nucleoli: Red. Eosinophilic granules: Pink purple Basophilic granules: Blue-purple to blue |
Helicobacter pylori is also stained. |
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| Naphthol AS-D chloroacetate esterase staining | Leukemic cell differentiation, monocyte differentiation, FAB classification |
Myelocytes: Blue granules | |||
SRL uses 20% formalin fixative for most of the staining.
