Laboratory:Akiruno

Laboratory:Akiruno
CODE:05434 4
Southern Blot Hybridization
A technique to detect a specific gene by the following procedures: After restriction enzyme digestion of DNA, DNA is isolated and denatured to a single-stranded form with electrophoresis. Using capillary action, the singlestranded DNA is transferred to a nylon membrane and hybridized with a target probe to detect the target gene. This technique is used for analysis of abnormal changes in DNA in a quantitative and qualitative manner.
TEST NAME | SPECIMEN | CONTAINER |
---|---|---|
Immunoglobulin L chain Jκrearrangement
|
cell
|
CAP COLOR | STORE | TURNAROUND |
---|---|---|
| 11-13 |
METHODOLOGY | REFERENCE RANGE |
---|---|
Southern Blot Hybridization Southern Blot Hybridization | Gene rearrangement not recognized |
£Blood/bone marrow fluid/cells: Avoid freezing. Please fill in the clinical diagnosis name, etc. on the request form. Please submit the cells stored and refrigerated in a culture container (filled with culture medium) used in the hospital. Since high-molecular DNA cannot be obtained from tissues fixed with formalin, etc., it is impossible to analyze them by Southern blot hybridization.