Immunoglobulin L chain Jκrearrangement

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gene-related test
immune-relevant genetic rearrangement tests
Immunoglobulin L chain J_κrearrangement

Laboratory：Akiruno

○Immunoglobulin L chain J_κrearrangement

CODE：05434 4

TEST NAME SPECIMEN
REQUIREMENT
（ｍL） CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT)
Immunoglobulin L chain J_κrearrangement
cell
2×107cells
cluture container
11-13 Southern Blot Hybridization
Southern Blot Hybridization
A technique to detect a specific gene by the following procedures: After restriction enzyme digestion of DNA, DNA is isolated and denatured to a single-stranded form with electrophoresis. Using capillary action, the singlestranded DNA is transferred to a nylon membrane and hybridized with a target probe to detect the target gene. This technique is used for analysis of abnormal changes in DNA in a quantitative and qualitative manner.
Gene rearrangement not recognized

TEST NAME	SPECIMEN REQUIREMENT （ｍL）	CONTAINER
Immunoglobulin L chain J_κrearrangement	cell 2×107cells	cluture container

CAP COLOR	STORE TEMPERATURE (STABILITY)	TURNAROUND TIME (DAY)
		11-13

METHODOLOGY	REFERENCE RANGE (UNIT)
Southern Blot Hybridization Southern Blot Hybridization A technique to detect a specific gene by the following procedures: After restriction enzyme digestion of DNA, DNA is isolated and denatured to a single-stranded form with electrophoresis. Using capillary action, the singlestranded DNA is transferred to a nylon membrane and hybridized with a target probe to detect the target gene. This technique is used for analysis of abnormal changes in DNA in a quantitative and qualitative manner.	Gene rearrangement not recognized

METHODOLOGY

REFERENCE RANGE
(UNIT)

Southern Blot Hybridization

Southern Blot Hybridization
A technique to detect a specific gene by the following procedures: After restriction enzyme digestion of DNA, DNA is isolated and denatured to a single-stranded form with electrophoresis. Using capillary action, the singlestranded DNA is transferred to a nylon membrane and hybridized with a target probe to detect the target gene. This technique is used for analysis of abnormal changes in DNA in a quantitative and qualitative manner.

Gene rearrangement not recognized

COMMENT

￡Blood/bone marrow fluid/cells: Avoid freezing. Please fill in the clinical diagnosis name, etc. on the request form. Please submit the cells stored and refrigerated in a culture container (filled with culture medium) used in the hospital. Since high-molecular DNA cannot be obtained from tissues fixed with formalin, etc., it is impossible to analyze them by Southern blot hybridization.

TEST DIRECTORY