TEST DIRECTORY

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Laboratory：Akiruno

○MLC (mixed lymphocyte culture)

CODE：05178 9

  • TEST NAME SPECIMEN
    REQUIREMENT
    （ｍL）     CONTAINER CAP COLOR STORE
    TEMPERATURE
    (STABILITY)     TURNAROUND
    TIME (DAY)     METHODOLOGY REFERENCE RANGE
    (UNIT)
  • Reservation Specified days
    MLC (mixed lymphocyte culture)
    (Recipient) Blood (heparin addition) and
    (Donor) Blood (heparin addition) and
    (Unrelated person) Blood (heparin addition)

    20.0, 10.0, and 10.0
    PH9 Room temperature
     9-11 3H-thymidine uptake ability

    3H-thymidine uptake ability (3H-TdR uptake) Method.
    Lymphocytes are cultured with a stimulating substance and 3H-thymidine, and the amount of 3H-thymidine taken into the cells by DNA synthesis is expressed as radioactivity. Measure. PHA, ConA, drugs, etc. are used as stimulants.

COMMENT


Acceptable days are Tuesday to Friday. Please be sure to clearly state the relationship, clinical diagnosis name, administered drug name, blood transfusion history, and transplant history. MLC cultures lymphocytes under sterile conditions, so avoid opening the collection container or transferring the sample. MLC unrelated blood will be used as a control.
About MLC (Mixed Lymphocyte Culture)
MLC (Mixed Lymphocyte Culture) is a pre-booked test, so please be sure to contact our sales staff before blood collection.
Collect 20.0mL (2 bottles) of recipient blood, 10.0mL (1 bottle) of donor blood, and 10.0mL (1 bottle) of unrelated blood into the container shown below, and mix well. Please store at room temperature.
MLC cultures lymphocytes under sterile conditions, so avoid opening the collection container or transferring the sample.
Please write the facility name and name on the label of the container.
If the number of lymphocytes is low, please collect more blood.

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