Laboratory：Akiruno
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- cellular immunity test
- *Lymphocyte surface marker analysis by two-color analysis
- Two-colorCD57/CD16
Laboratory：Akiruno
○Two-colorCD57/CD16
CODE：05045 6
-
TEST NAME
SPECIMEN
REQUIREMENT
（ｍL） CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT) -
Two-colorCD57/CD16
Blood (heparin added)
3.0
PH5
3-5 Two-color flow cytometryCD57(-)CD16(+)1.0-15.0Flow cytometry
A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen).
Two-color flow cytometry uses a combination of fluorochromes for double staining.
CD57(-)CD16(-)49.0-87.0
CD57(+)CD16(-)2.0-40.0
CD57(+)CD16(+)2.0-27.0(%)
COMMENT
Acceptable days are Monday to Friday.
Please see below for the list of implementation items.
If you request 10 or more items, please collect 5.0mL of blood.
About requesting a cell-mediated immunity test
1. Please submit the specimen on the same day after collection.
2. If there are few lymphocytes, please collect more blood.
3. If requesting bone marrow fluid, please use a special container (H00) and submit it refrigerated. However, for CD34 quantitative, if it is diluted with storage solution, correct test results will not be obtained, so please use a special container (PH5).
4. If you are making a request for a tissue such as lymph node, please suspend it in a special container (H20) and submit it in a refrigerator.
Collect blood into the container shown below, mix well, and store at room temperature.
(For 10 or more items, please collect 5.0mL of blood.)
After collecting the specimen, please submit it on the same day.
CONTAINER
PH5 旧容器記号 G 12
ヘパリン入り (真空採血量5mL)
内容：ヘパリンNa 65IU
貯蔵方法：室温
有効期間：製造から2年
supplementary information
Two-color解析によるリンパ球表面マーカー検査
| 項目コード 新項目コード |
検査項目 JLAC10 |
基準値 (%) | 実施料 判断料 |
|---|---|---|---|
|
5040 1
7841 0
017604401
358101
3581
|
Two-color CD4/CD45RA 5I700-0000-019-662 |
CD4 (-) CD45RA (+) 30.0～57.0 CD4 (-) CD45RA (-) 5.0～23.0 CD4 (+) CD45RA (-) 15.0～34.0 CD4 (+) CD45RA (+) 9.0～37.0 |
185 ※6 |
|
5041 9
7842 9
017614401
358201
3582
|
Two-color CD4/CD29 5I701-0000-019-662 |
CD4 (-) CD29 (+) 22.0～59.0 CD4 (-) CD29 (-) 12.0～38.0 CD4 (+) CD29 (-) 9.0～34.0 CD4 (+) CD29 (+) 11.0～26.0 |
|
|
5042 6
7843 8
017624401
358801
3588 |
Two-color CD11b/CD8 5I710-0000-019-662 |
CD11b (-) CD8 (+) 11.0～40.0 CD11b (-) CD8 (-) 29.0～64.0 CD11b (+) CD8 (-) 8.0～32.0 CD11b (+) CD8 (+) 6.0～27.0 CD8/BRIGHT 20.0以下 CD8/DULL 3.0～17.0 |
|
|
5043 3
4056 5
017634401
358601
3586
|
Two-color HLA-DR/CD4 5I706-0000-019-662 |
HLA-DR (-) CD4 (+) 22.0～51.0 HLA-DR (-) CD4 (-) 24.0～61.0 HLA-DR (+) CD4 (-) 8.0～37.0 HLA-DR (+) CD4 (+) 1.0～12.0 |
|
|
5044 1
4046 6
017644401
358701
3587
|
Two-color HLA-DR/CD8 5I707-0000-019-662 |
HLA-DR (-) CD8 (+) 15.0～49.0 HLA-DR (-) CD8 (-) 28.0～62.0 HLA-DR (+) CD8 (-) 6.0～25.0 HLA-DR (+) CD8 (+) 2.0～27.0 |
|
|
5045 8
7833 9
017654401
359101
3591
|
Two-color CD57/CD16 5I721-0000-019-662 |
CD57 (-) CD16 (+) 1.0～15.0 CD57 (-) CD16 (-) 49.0～87.0 CD57 (+) CD16 (-) 2.0～40.0 CD57 (+) CD16 (+) 2.0～27.0 |
|
|
2372 8
7834 8
015604401
359301
3593
|
Two-color CD16/CD56 5I724-0000-019-662 |
CD16 (-) CD56 (+) 2.0～20.0 CD16 (-) CD56 (-) 57.0～91.0 CD16 (+) CD56 (-) 9.0以下 CD16 (+) CD56 (+) 4.0～33.0 |
|
|
2394 3
7838 4
015624401
358401
3584
|
Two-color CD45RO/CD4 5I703-0000-019-662 |
CD45RO (-) CD4 (+) 6.0～34.0 CD45RO (-) CD4 (-) 28.0～58.0 CD45RO (+) CD4 (-) 7.0～32.0 CD45RO (+) CD4 (+) 10.0～32.0 |
185 ※6 |
|
2748 0
3893 7
016684401
384301
8748
|
Two-color CD4/CD8 5I702-0000-019-662 |
CD4 (-) CD8 (+) 22.0～54.0 CD4 (-) CD8 (-) 14.0～38.0 CD4 (+) CD8 (-) 23.0～52.0 CD4 (+) CD8 (+) 7.0以下 CD4/CD8比 0.40～2.30 |
|
|
5662 9
7845 6
018724401
358501
3585
|
Two-color HLA-DR/CD3 5I705-0000-019-662 |
HLA-DR (-) CD3 (+) 33.0～75.0 HLA-DR (-) CD3 (-) 7.0～36.0 HLA-DR (+) CD3 (-) 3.0～18.0 HLA-DR (+) CD3 (+) 5.0～33.0 |
|
|
5665 1
6215 6
01874A101
359501
3595
|
Two-color CD4/CD25 5I751-0000-019-662 |
CD4 (-) CD25 (+) 2.0～14.0 CD4 (-) CD25 (-) 37.0～69.0 CD4 (+) CD25 (-) 15.0～39.0 CD4 (+) CD25 (+) 6.0～21.0 |
|
|
5670 0
7781 7
018754401
358901
3589
|
Two-color CD20/CD5 5I715-0000-019-662 |
CD20 (-) CD5 (+) 54.0～87.0 CD20 (-) CD5 (-) 9.0～40.0 CD20 (+) CD5 (-) 9.0以下 CD20 (+) CD5 (+) 13.0以下 |
