FoundationOne CDx Cancer Genome Profile

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gene-related test
genetic tests for malignant tumor (solid tumor)
FoundationOne CDx Cancer Genome Profile

Laboratory：Akiruno

○FoundationOne CDx Cancer Genome Profile

CODE：03977 4

TEST NAME SPECIMEN
REQUIREMENT
（ｍL） CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT)
FoundationOne CDx Cancer Genome Profile
Unstained specimen slide
and
HE stained slide

10 sheets, thickness 4 to 5μm
and
1 sheet
Z10
16-19 Next Generation Sequencing (NGS)
A method that uses a next-generation sequencer to simultaneously determine the base sequences of a huge number of DNA fragments.

COMMENT

The optimal percentage of nucleated tumor cells on an unstained specimen slide is 30% or more for the area after macrodissection, but please submit at least 20%. Furthermore, since the amount of DNA in hepatocytes is twice that of other somatic cells, a higher proportion of tumor cells is required if the specimen is liver tissue. Please see below for points to note when submitting unstained specimen slides.
Please avoid duplicate requests with other items. This testing method increases the influence of contamination, so please be careful when handling the specimen. The number of days required may vary depending on the measurement and analysis situation.
●Notes on submission
・Do not perform acid decalcification. If demineralization is required, use a neutral demineralization solution containing EDTA as the main ingredient.
・When the surface area of the tissue is less than 25 mm²: The number of sections with a thickness of 4 to 5 μm is determined so that the total volume of the sections is 1 mm³ or more. Please add it.
●About unstained specimen slides
Immediately immerse the collected tissue in a 10% neutral buffered formalin solution to fix it (recommended fixation time is 6 to 48 hours). When submitting, please prepare serial sections at the specified thickness from formalin-fixed, paraffin-embedded FFPE (FFPE) blocks prepared within the past 3 years, if possible. Please be careful to avoid contamination by changing the microtome blade for each specimen when slicing. Please note that as nucleic acids are fragmented due to formalin fixation of tissues, analysis may not be possible depending on the type and composition of the fixative, fixation time, and storage condition of the specimen after fixation. .
●About biopsy specimens
Biopsy specimens often contain only a small amount of specimen, and there is a possibility that most of the tissue itself has disappeared or that the tissue has become a piece of tissue that does not contain tumor cells. Please be aware of this in advance.

CONTAINER

Z10 旧容器記号 t 30

[オブジェクトケース]
プレパラート (スライドグラス)

貯蔵方法：室温

TEST DIRECTORY