FoundationOne CDx Cancer Genome Profile

TOP
gene-related test
genetic tests for malignant tumor (solid tumor)
FoundationOne CDx Cancer Genome Profile

Laboratory：Akiruno

○FoundationOne CDx Cancer Genome Profile

CODE：03977 4

TEST NAME SPECIMEN
REQUIREMENT
（ｍL） CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT)
FoundationOne CDx Cancer Genome Profile
Unstained specimen slide
and
HE stained slide
(Unstained specimen slide)10 pieces 4-5 μm thick
and
(HE stained slide)1 piece
Z10
16-19 Next Generation Sequencing (NGS)
A method that uses a next-generation sequencer to simultaneously determine the base sequences of a huge number of DNA fragments.

COMMENT

The optimal percentage of nucleated tumor cells on the unstained specimen slide is 30% or more for the area after macrolysis, but please submit at least 20%. Furthermore, since the amount of DNA in hepatocytes is twice that of other somatic cells, a higher proportion of tumor cells is required if the specimen is liver tissue. Please see below for points to note when submitting unstained specimen slides.
Please avoid duplicate requests with other items. This testing method can be affected by contamination, so please be careful when handling the specimen. The number of days required may vary depending on the measurement and analysis situation.
●Notes on submission
・Do not perform acid decalcification. If decalcification is required, use a neutral decalcification solution containing EDTA as the main ingredient.
Add a number of 4-5 μm thick sections so that the total volume of the sections is 1mm ³ or more.
●About unstained specimen slides
Immediately immerse the collected tissue in a 10% neutral buffered formalin solution and fix it (recommended fixation time is 6 to 48 hours). When submitting your application, please prepare serial sections at the specified thickness from a formalin-fixed, paraffin-embedded (FFPE) block produced within the past 3 years, if possible. When thinly slicing, please be careful about contamination by changing the microtome blade for each sample. Additionally, please note that since nucleic acids are fragmented due to formalin fixation of tissues, analysis may not be possible depending on the type and composition of the fixative, fixation time, and storage condition of the specimen after fixation.
●About biopsy specimens
Biopsy specimens often contain only a small amount of specimen, and there is a possibility that most of the tissue itself has disappeared or that the tissue has become a piece of tissue that does not contain tumor cells. Please be aware of this in advance.

CONTAINER

Z10 旧容器記号 t 30

[オブジェクトケース]
プレパラート (スライドグラス)

貯蔵方法：室温

TEST DIRECTORY