Microsatellite instability (MSI) testing (Lynch syndrome)

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gene-related test
genetic tests
Microsatellite instability (MSI) testing (Lynch syndrome)

Laboratory：Akiruno

○Microsatellite instability (MSI) testing (Lynch syndrome)

CODE：03894 6

TEST NAME SPECIMEN
REQUIREMENT
（ｍL） CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT)
Microsatellite instability (MSI) testing (Lynch syndrome)
Unstained specimen slide
5 to 10 sheets
thickness 5μm
Z10
8-14 Multiplex PCR-fragment analysis
After amplifying multiple target genes, the PCR amplification products are subjected to capillary electrophoresis using a sequencer.
A method that separates DNA according to its size and analyzes and identifies peaks according to the amount of amplification using the QMVR width set by dedicated software.

COMMENT

Acceptable days are from Monday to Friday.
Five types of markers (BAT25, BAT26, NR21, NR24, MONO27) will be analyzed and determined. avoid duplicate requests in one sample.
The percentage of tumor cells required for testing is at least 50%. See below for the notes on the submission of unstained specimen slides.
● Submission Conditions
Unstained specimen slides should be histopathologically evaluated and confirmed to contain at least the required percentage of tumor cells (tumor cells as a percentage of total cells in the specimen) for the examination. If the required percentage is not met, mark the tumor cell area from the reverse side of the unstained specimen slides; otherwise, macrodissection may failure, which may affect the results such as false negative. For the microsatellite-instability (MSI) test (FFPE) and microsatellite-instability (MSI) test (Lynch syndrome), be sure to mark the tumor cell area, as it is necessary to distinguish between the tumor and normal areas due to the nature of the test.
●About submission of unstained specimen slides
Immediately immerse the collected tissue in a 10% neutral buffered formalin solution and fix it (recommended fixation time is 6 to 48 hours). For submission, prepare serial sections of the specified thickness from formalin-fixed, paraffin-embedded (FFPE) blocks made within 3 years whenever possible.When thin sectioning, be careful to avoid contamination by replacing the microtome blade for each specimen. Also, since nucleic acids are fragmented by formalin fixation of tissues, analysis may not be possible depending on the type and composition of the fixative solution, fixation time, and storage conditions of the specimens after fixation.
● About biopsy specimens
Note that biopsy specimens are often minute amounts of specimens and may contain almost no tissue itself or tissue fragments that do not contain tumor cells.

CONTAINER

Z10 旧容器記号 t 30

[オブジェクトケース]
プレパラート (スライドグラス)

貯蔵方法：室温

TEST DIRECTORY