Microsatellite instability (MSI) testing (Lynch syndrome)

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gene-related test
genetic tests
Microsatellite instability (MSI) testing (Lynch syndrome)

Laboratory：Akiruno

○Microsatellite instability (MSI) testing (Lynch syndrome)

CODE：03894 6

TEST NAME SPECIMEN
REQUIREMENT
（ｍL） CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT)
Microsatellite instability (MSI) testing (Lynch syndrome)
Unstained specimen slide
5-10 pieces 5μm
Z10
8-14 Multiplex PCR-fragment analysis
After amplifying multiple target genes, PCR amplification products are subjected to capillary electrophoresis on a sequencer.
A method in which DNA is separated according to its size, and peaks corresponding to the amplified amount are analyzed and identified by the QMVR width set by dedicated software.

COMMENT

Acceptable days are Monday to Friday.
We will analyze and judge 5 types of markers (BAT25, BAT26, NR21, NR24, MONO27). Please avoid duplicate requests with other items.
The percentage of tumor cells required for testing is 50% or more. Please see below for points to note when submitting unstained specimen slides.
●Submission conditions
Unstained specimen slides should be histopathologically evaluated to ensure that the proportion of tumor cells present is at least the amount required for the test. If the tumor cell percentage is less than adequate, mark the tumor cell area from the back of the unstained specimen slide. Please note that if you submit your application without marking, macrodissection may not be possible and this may affect the results, such as false negatives.
In addition, in the two items of microsatellite instability (MSI) test (FFPE) and microsatellite instability (MSI) test (Lynch syndrome), due to the characteristics of the test, it is necessary to distinguish between tumor areas and normal areas. Please be sure to mark the tumor cell area.
●About unstained specimen slides
Immediately immerse the collected tissue in a 10% neutral buffered formalin solution and fix it (recommended fixation time is 6 to 48 hours). When submitting, please prepare serial sections at the specified thickness from formalin-fixed paraffin-embedded (FFPE) blocks produced within the past 3 years if possible. When thinly slicing, please be careful about contamination by changing the microtome blade for each sample. Additionally, please note that since nucleic acids are fragmented due to formalin fixation of tissues, analysis may not be possible depending on the type and composition of the fixative, fixation time, and storage condition of the specimen after fixation. .
●About biopsy specimens
Biopsy specimens often contain only a small amount of specimen, and there is a possibility that most of the tissue itself has disappeared or that

CONTAINER

Z10 旧容器記号 t 30

[オブジェクトケース]
プレパラート (スライドグラス)

貯蔵方法：室温

TEST DIRECTORY