TEST DIRECTORY

0 selected

Laboratory：Akiruno

○Microsatellite instability (MSI) testing (FFPE)

CODE：03890 0

  • TEST NAME SPECIMEN
    REQUIREMENT
    （ｍL）     CONTAINER CAP COLOR STORE
    TEMPERATURE
    (STABILITY)     TURNAROUND
    TIME (DAY)     METHODOLOGY REFERENCE RANGE
    (UNIT)
  • Microsatellite instability (MSI) testing (FFPE)
    Unstained specimen slide
    5-10 pieces 5μm
    Z10 Room temperature
     4-8 Multiplex PCR-fragment analysis

    After amplifying multiple target genes, PCR amplification products are subjected to capillary electrophoresis on a sequencer.
    A method in which DNA is separated according to its size, and peaks corresponding to the amplified amount are analyzed and identified by the QMVR width set by dedicated software.

COMMENT


We will analyze and determine regarding five types of markers (BAT25, BAT26, NR21, NR24, MONO27). Please avoid duplicate requests with other items.
The percentage of tumor cells required for the test is 50% or more. Please see below for notes on submitting unstained specimen slides.
●Submission Conditions
Confirm that the unstained specimen slide has been histopathologically evaluated and that tumor cells are present in at least the required percentage for the test. If the tumor cell percentage is not met, mark the tumor cell area from the back of the unstained specimen slide.  Please note that if the specimens are submitted without marking, macrodissection cannot be performed, which may affect the determination results, such as being a false negative result.
For the microsatellite-instability (MSI) test (FFPE) and microsatellite-instability (MSI) test (Lynch syndrome), please be sure to mark the tumor cell area, as it is necessary to distinguish between the tumor and normal areas due to the nature of the test.
●About Unstained Specimen Slides
The collected tissue should be immediately immersed in 10% neutral buffered formalin solution for fixation (recommended fixation time: 6-48 hours). When submitting, please prepare serial sections with the specified thickness from formalin-fixed, paraffin-embedded (FFPE) blocks prepared within the last 3 years to the extent possible. Please be careful to avoid contamination by changing the microtome blade for each specimen during thin slicing, for example. In addition, please note that since nucleic acids are fragmented due to formalin fixation of tissues, analysis may be impossible depending on the type and composition of the fixation solution, fixation time, and preservative conditions of the specimens after fixation.
●About Biopsy Specimens
Please note that since biopsy specimens are often minute amounts, the tissue itself may be almost lost or may be a piece of tissue that contains no tumor

CONTAINER

Notifications of URL changes/lab information added

ラボを選択してください

Choose a laboratory

Notifications of URL changes/lab information added

Laboratory List

Advanced search