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Laboratory：Akiruno

○Microsatellite instability (MSI) testing (FFPE)

CODE：03890 0

  • TEST NAME SPECIMEN
    REQUIREMENT
    （ｍL）     CONTAINER CAP COLOR STORE
    TEMPERATURE
    (STABILITY)     TURNAROUND
    TIME (DAY)     METHODOLOGY REFERENCE RANGE
    (UNIT)
  • Microsatellite instability (MSI) testing (FFPE)
    Unstained specimen slide
    5 to 10 sheets
    thickness 5μm
    Z10 Room temperature
     4-8 Multiplex PCR-fragment analysis

    After amplifying multiple target genes, the PCR amplification products are subjected to capillary electrophoresis using a sequencer.
    A method that separates DNA according to its size and analyzes and identifies peaks according to the amount of amplification using the QMVR width set by dedicated software.

COMMENT


It is analyzed and determined for 5 types of markers (BAT25, BAT26, NR21, NR24, and MONO27). Avoid duplicate requests in one sample.
The percentage of tumor cells required for testing is 50% or more.
See below for notes on submitting unstained specimen slides.
●Submission Conditions
Unstained specimen slides should be histopathologically evaluated to ensure that the required percentage of tumor cells (tumor cells as a percentage of total cells in the specimen) is present. If the required percentage is not met, mark the tumor cell area from the back of the unstained specimen slide.  Note that submission of unstained specimen slides without marking may result in failure of macrodissection, which may affect the results, such as a false negative result.
In addition, for the microsatellite instability (MSI) test (FFPE) and microsatellite instability (MSI) test (Lynch syndrome), it is necessary to distinguish between the tumor and normal areas due to the nature of the test, thus be sure to mark the tumor cell area.
●About unstained specimen slides
Immediately immerse the collected tissue in a 10% neutral buffered formalin solution and fix it (recommended fixation time is 6 to 48 hours). When submitting, prepare serial sections at the specified thickness from formalin-fixed paraffin-embedded (FFPE) blocks prepared within the last 3 years, if possible. Be careful to avoid contamination by changing the microtome blade for each specimen when slicing. Please note that as nucleic acids are fragmented due to formalin fixation of tissues, analysis may not be possible depending on the type and composition of the fixative, fixation time, and storage condition of the specimen after fixation.
●About biopsy specimens
Biopsy specimens often contain only a small amount of specimen, and there is a possibility that most of the tissue itself has disappeared or that the tissue has become a piece of tissue that does not contain tumor cells. Be aware of this in advance.

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