TEST DIRECTORY

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Laboratory：Akiruno

○ArcherMETex14 Skipping (FFPE)（Suspended beyond orders placed 06-13-2023）

CODE：02874 9

  • TEST NAME SPECIMEN
    REQUIREMENT
    （ｍL）     CONTAINER CAP COLOR STORE
    TEMPERATURE
    (STABILITY)     TURNAROUND
    TIME (DAY)     METHODOLOGY REFERENCE RANGE
    (UNIT)
  • INACTIVE
    ArcherMETex14 Skipping (FFPE)（Suspended beyond orders placed 06-13-2023）
    Unstained specimen slide
    Z10 Room temperature
     8-14 Next Generation Sequencing (NGS)

    A method that uses a next-generation sequencer to simultaneously determine the base sequences of a huge number of DNA fragments.

COMMENT


The purpose of this study is to analyze MET ex14 skipping in RNA extracted from cancer tissue to assist in determining the indication of the therapeutic drug Tepotinib for patients with non-small cell lung cancer.
The percentage of tumor cells required for testing is 10% or more. Please see below for notes on submitting unstained specimen slides.
Please avoid duplicate requests with other items. Since this test method is more susceptible to contamination, please handle the specimens with care when collecting them.

Please submit at least 10 slides. Specimens with a low number of nucleated cells may not be tested because the required amount of nucleic acid cannot be obtained.
If the biopsy specimen is small (the area of the tissue section is 4mm2 or less [2mm × 2mm or less]), please submit 15 or more specimens.

●About submission conditions
Unstained specimen slides should be histopathologically evaluated to ensure that tumor cells are present in a proportion greater than that required for testing. If the tumor cell percentage is not met, mark the tumor cell area from the back of the unstained specimen slide. Please be aware that if the specimens are submitted without   marking, macrodissection cannot be performed, which may affect the results, such as a false negative result.
●About unstained specimen slides
The collected tissue should be immediately immersed in a 10% neutral buffered formalin solution for fixation (Recommended immobilization time is 18-36 hours for surgical materials and 3-6 hours for biopsy materials). When submitting, please prepare serial sections of the specified thickness from formalin-fixed, paraffin-embedded (FFPE) blocks made within the last three years if possible. Please be careful to avoid contamination by changing the microtome blade for each specimen during thin sectioning. Additionally, Please note that since nucleic acids are fragmented by formalin fixation of tissues, analys

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