Laboratory：Akiruno
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- Multiple myeloma multiparameter flow cytometry (8color)
Laboratory：Akiruno
○Multiple myeloma multiparameter flow cytometry (8color)
CODE：02829 9
-
TEST NAME
SPECIMEN
REQUIREMENT
（ｍL） CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT) -
Multiple myeloma multiparameter flow cytometry (8color)
bone marrow aspirate (EDTA-2Na added)
2.0
PN2,PN5
4-6 Flow cytometry(FCM)Flow cytometry
A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen).
Two-color flow cytometry uses a combination of fluorochromes for double staining.
COMMENT
Please collect the bone marrow fluid into a blood collection tube containing EDTA-2Na and submit it at room temperature.
Consignment days are Monday to Friday. Please see below for implementation items. Please avoid duplicate requests with other items.
●About requesting multiple myeloma multiparameter flow cytometry (8color)
1. After collecting the specimen, please submit it on the same day.
2. Collect bone marrow fluid into a blood collection tube containing EDTA-2Na and submit at room temperature.
3. This item allows analysis of more than 10 million white blood cells using 8-color multiparameter flow cytometry for patients with multiple myeloma.
Collect the 2.0mL of bone marrow fluid, mix well in the designated container (PN5 or PN2), and store at room temperature. Please submit the specimen on the same day after collection.
CONTAINER
PN2 旧容器記号 C C3 4
EDTA-2Na入り (真空採血量2mL)
内容：EDTA-2Na 3.0mg
貯蔵方法：室温
有効期間：製造から2年
PN5 旧容器記号 C C3 4
EDTA-2Na入り (真空採血量5mL)
内容：EDTA-2Na 7.5mg
貯蔵方法：室温
有効期間：製造から2年
supplementary information
白血病・リンパ腫解析検査 (LLA) ・悪性リンパ腫解析検査 (MLA)
多発性骨髄腫解析検査CD38マルチ解析
多発性骨髄腫マルチパラメーターフローサイトメトリー (8color)
悪性リンパ腫解析検査7AAD解析
| 項目 | 検出抗体 |
|---|---|
| 白血病・リンパ腫解析検査 (LLA) | CD1a CD2 CD3 CD4 CD5 CD7 CD8 CD10 CD13 CD14 CD19 CD20 CD33 CD34 CD41 CD56 GP-A HLA-DR |
| 悪性リンパ腫解析検査 (MLA) | CD2 CD3 CD4 CD5 CD7 CD8 CD10 CD11c CD16 CD19 CD20 CD23 CD25 CD30 CD34 CD56 κ-chain λ-chain |
| 多発性骨髄腫解析検査CD38マルチ解析 | CD7 CD19 CD20 CD33 CD45 CD49e CD54 CD56 CD138 MPC-1 cy κ-chain cy λ-chain |
| 多発性骨髄腫マルチパラメーターフローサイトメトリー (8color) | CD19 CD27 CD38※ CD45 CD56 CD138 cyIgκ cyIgλ |
| 悪性リンパ腫解析検査7AAD解析 | CD2 CD3 CD4 CD5 CD7 CD8 CD10 CD19 CD20 CD25 CD30 CD34 CD38 CD45 CD56 IgM κ-chain λ-chain |
※マルチエピトープ抗体を使用しております。
