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Laboratory:Akiruno

Malignant lymphoma analysis test (MLA) CD45 gating (hematopoietic malignant tumor cell test)

  • TEST NAME SPECIMEN
    REQUIREMENT
    (mL)
    CONTAINER CAP COLOR STORE
    TEMPERATURE
    (STABILITY)
    TURNAROUND
    TIME (DAY)
    METHODOLOGY REFERENCE RANGE
    (UNIT)
  • Specified days
    Malignant lymphoma analysis test (MLA) CD45 gating (hematopoietic malignant tumor cell test)
    lymph nodes
    5×5×5mm
    H20 Refrigeration
    3-5 Flow cytometry(FCM)

    Flow cytometry
    A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen).
    Two-color flow cytometry uses a combination of fluorochromes for double staining.

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COMMENT


Avoid freezing. Acceptable days are Monday through Friday. Please see below for the items to be performed.
About Requests for Cellular Immunity Tests
1. Submit the specimen on the same day after collection.
2. If the number of lymphocytes is low, collect extra amount of blood.
3. If you request with bone marrow fluid, please use the special container (H00) and submit it under refrigeration. However, since the CD34 quantitative cannot yield accurate test results if diluted with the preservation solution, please use a special container (PH5).
4. If you request with tissues such as lymph nodes, please submit them floated in a special container (H20) under refrigeration.
Float a 5 x 5 x 5 mm lymph node in the designated container and preserve it refrigerated. Submit the specimen on the same day after collection.

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