Laboratory:Akiruno
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- viral infection test
- viral infection test
- EB virus DNA (Clonality)
Laboratory:Akiruno
○EB virus DNA (Clonality)
CODE:02494 1
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TEST NAME
SPECIMEN
REQUIREMENT
(mL) CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT) -
EB virus DNA (Clonality)
Blood (EDTA-2Na added)
7.0
PN7
(10 days)
17-23 Southern Blot HybridizationSouthern blot hybridization
A method in which DNA digested with restriction enzymes is fractionated by electrophoresis, denatured into single-stranded DNA, transferred onto a nylon membrane using capillary action, and hybridized with a target probe to detect the target gene. It is used to analyze abnormal quantitative and qualitative changes in DNA.
COMMENT
Blood: Avoid cryopreservation. Please note that if the number of cells has decreased due to chemotherapy, etc., it may not be possible to extract the required amount of DNA. Since high-molecular DNA cannot be obtained from tissues fixed with formalin, etc., it is impossible to analyze them by Southern blot hybridization. With this method, EBV DNA may not be detected if the amount of viral DNA is small.
Please note that if you request multiple samples at the same time, the number of days required may vary.
Collect blood into the container shown below, mix well, and store in the refrigerator.
CONTAINER
PN7 旧容器記号 A 4
EDTA-2Na入り (真空採血量7mL)
内容:EDTA-2Na 10.5mg
貯蔵方法:室温
有効期間:製造から2年