Laboratory：Akiruno
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- cellular immunity test
- *Lymphocyte surface marker analysis by two-color analysis
- Two-colorCD45RO/CD4
Laboratory：Akiruno
○Two-colorCD45RO/CD4
CODE：02394 2
-
TEST NAME
SPECIMEN
REQUIREMENT
（ｍL） CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT) -
Two-colorCD45RO/CD4
Blood (heparin added)
3.0
PH5
3-5 Two-color flow cytometryCD45RO(-)CD4(+)6.0-34.0Flow cytometry
A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen).
Two-color flow cytometry uses a combination of fluorochromes for double staining.
CD45RO(-)CD4(-)28.0-58.0
CD45RO(+)CD4(-)7.0-32.0
CD45RO(+)CD4(+)10.0-32.0(%)
TEST NAME | SPECIMEN | CONTAINER |
|---|---|---|
|
Blood (heparin added)
|
CAP COLOR | STORE | TURNAROUND |
|---|---|---|
|
| 3-5 |
METHODOLOGY | REFERENCE RANGE |
|---|---|
Two-color flow cytometry Flow cytometry |
CD45RO(-)CD4(+)6.0-34.0 |
COMMENT
Acceptable days are Monday to Friday.
Please see below for the list of implementation items.
If you request 10 or more items, please collect 5.0mL of blood.
About requesting a cell-mediated immunity test
1. Please submit the specimen on the same day after collection.
2. If there are few lymphocytes, please collect more blood.
3. If requesting bone marrow fluid, please use a special container (H00) and submit it refrigerated. However, for CD34 quantitative, if it is diluted with storage solution, correct test results will not be obtained, so please use a special container (PH5).
4. If you are making a request for a tissue such as lymph node, please suspend it in a special container (H20) and submit it in a refrigerator.
Collect blood into the container shown below, mix well, and store at room temperature.
(For 10 or more items, please collect 5.0mL of blood.)
After collecting the specimen, please submit it on the same day.
CONTAINER
PH5 Previous container symbol G
With heparin (5mL vacuum blood sampling tube)
Contents: Heparin sodium 65IU
Storage method: Room temperature
Expiration period: 2 years from production
supplementary information
Lymphocyte surface marker analysis by two-color analysis
| CODE | TEST NAME | Reference range (%) |
|---|---|---|
05040 1
|
Two-color CD4/CD45RA |
CD4 (-) CD45RA (+) 30.0 – 57.0 CD4 (-) CD45RA (-) 5.0 – 23.0 CD4 (+) CD45RA (-) 15.0 – 34.0 CD4 (+) CD45RA (+) 9.0 – 37.0 |
05041 0
|
Two-color CD4/CD29 |
CD4 (-) CD29 (+) 22.0 – 59.0 CD4 (-) CD29 (-) 12.0 – 38.0 CD4 (+) CD29 (-) 9.0 – 34.0 CD4 (+) CD29 (+) 11.0 – 26.0 |
05042 9
|
Two-color CD11b/CD8 |
CD11b (-) CD8 (+) 11.0 – 40.0 CD11b (-) CD8 (-) 29.0 – 64.0 CD11b (+) CD8 (-) 8.0 – 32.0 CD11b (+) CD8 (+) 6.0 – 27.0 CD8/BRIGHT ≤ 20.0 CD8/DULL 3.0 ～ 17.0 |
05043 8
|
Two-color HLA-DR/CD4 |
HLA-DR (-) CD4 (+) 22.0 – 51.0 HLA-DR (-) CD4 (-) 24.0 – 61.0 HLA-DR (+) CD4 (-) 8.0 – 37.0 HLA-DR (+) CD4 (+) 1.0 – 12.0 |
05044 7
|
Two-color HLA-DR/CD8 |
HLA-DR (-) CD8 (+) 15.0 – 49.0 HLA-DR (-) CD8 (-) 28.0 – 62.0 HLA-DR (+) CD8 (-) 6.0 – 25.0 HLA-DR (+) CD8 (+) 2.0 – 27.0 |
05045 6
|
Two-color CD57/CD16 |
CD57 (-) CD16 (+) 1.0 – 15.0 CD57 (-) CD16 (-) 49.0 – 87.0 CD57 (+) CD16 (-) 2.0 – 40.0 CD57 (+) CD16 (+) 2.0 – 27.0 |
02372 6
|
Two-color CD16/CD56 |
CD16 (-) CD56 (+) 2.0 – 20.0 CD16 (-) CD56 (-) 57.0 – 91.0 CD16 (+) CD56 (-) ≤ 9.0 CD16 (+) CD56 (+) 4.0 – 33.0 |
02394 2
|
Two-color CD45RO/CD4 |
CD45RO (-) CD4 (+) 6.0 – 34.0 CD45RO (-) CD4 (-) 28.0 – 58.0 CD45RO (+) CD4 (-) 7.0 – 32.0 CD45RO (+) CD4 (+) 10.0 – 32.0 |
02748 9
|
Two-color CD4/CD8 |
CD4 (-) CD8 (+) 22.0 – 54.0 CD4 (-) CD8 (-) 14.0 – 38.0 CD4 (+) CD8 (-) 23.0 – 52.0 CD4 (+) CD8 (+) ≤ 7.0 CD4/CD8 ratio 0.40 – 2.30 |
05662 1
|
Two-color HLA-DR/CD3 |
HLA-DR (-) CD3 (+) 33.0 – 75.0 HLA-DR (-) CD3 (-) 7.0 – 36.0 HLA-DR (+) CD3 (-) 3.0 – 18.0 HLA-DR (+) CD3 (+) 5.0 – 33.0 |
05665 8
|
Two-color CD4/CD25 |
CD4 (-) CD25 (+) 2.0 – 14.0 CD4 (-) CD25 (-) 37.0 – 69.0 CD4 (+) CD25 (-) 15.0 – 39.0 CD4 (+) CD25 (+) 6.0 – 21.0 |
05670 2
|
Two-color CD20/CD5 |
CD20 (-) CD5 (+) 54.0 – 87.0 CD20 (-) CD5 (-) 9.0 – 40.0 CD20 (+) CD5 (-) ≤ 9.0 CD20 (+) CD5 (+) ≤ 13.0 |
