TEST DIRECTORY

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Laboratory:Akiruno

Immunoglobulin L chain Cλrearrangement

  • TEST NAME SPECIMEN
    REQUIREMENT
    (mL)
    CONTAINER CAP COLOR STORE
    TEMPERATURE
    (STABILITY)
    TURNAROUND
    TIME (DAY)
    METHODOLOGY REFERENCE RANGE
    (UNIT)
  • Immunoglobulin L chain Cλrearrangement
    tissue
    250mg
    ARR Freeze
    11-13 Southern Blot Hybridization

    Southern Blot Hybridization
    A technique to detect a specific gene by the following procedures: After restriction enzyme digestion of DNA, DNA is isolated and denatured to a single-stranded form with electrophoresis. Using capillary action, the singlestranded DNA is transferred to a nylon membrane and hybridized with a target probe to detect the target gene. This technique is used for analysis of abnormal changes in DNA in a quantitative and qualitative manner.

    Gene rearrangement not recognized

COMMENT


£Blood/bone marrow fluid/cells: Avoid freezing. Please fill in the clinical diagnosis name, etc. on the request form. Please submit the cells stored and refrigerated in a culture container (filled with culture medium) used in the hospital. Since high-molecular DNA cannot be obtained from tissues fixed with formalin, etc., it is impossible to analyze them by Southern blot hybridization.

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