Laboratory:Akiruno
- TOP
- gene-related test
- immune-relevant genetic rearrangement tests
- Immunoglobulin L chain Cκreconstitution
Laboratory:Akiruno
○Immunoglobulin L chain Cκreconstitution
CODE:02305 0
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TEST NAME
SPECIMEN
REQUIREMENT
(mL) CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT) -
Immunoglobulin L chain Cκreconstitution
Blood (EDTA-2Na added)
7.0
PN7
11-13 Southern Blot HybridizationGene rearrangement not recognizedSouthern blot hybridization
A method in which DNA digested with restriction enzymes is fractionated by electrophoresis, denatured into single-stranded DNA, transferred onto a nylon membrane using capillary action, and hybridized with a target probe to detect the target gene. It is used to analyze abnormal quantitative and qualitative changes in DNA.
COMMENT
Blood/bone marrow fluid/cells: Avoid cryopreservation. Please fill in the clinical diagnosis name, etc. on the request form. Please submit the cells stored and refrigerated in a culture container (filled with culture medium) used in the hospital. Since high-molecular DNA cannot be obtained from tissues fixed with formalin, etc., it is impossible to analyze them by Southern blot hybridization.
Collect blood into the container shown below, mix well, and store in the refrigerator.
CONTAINER
PN7 旧容器記号 A 4
EDTA-2Na入り (真空採血量7mL)
内容:EDTA-2Na 10.5mg
貯蔵方法:室温
有効期間:製造から2年