TEST DIRECTORY

VP6 (Previous container symbol W6) handling methods of samples

SAMPLING METHOD

1. Gently remove excess mucus from the vaginal area using a swab.
2. Insert the specimen collection swab into the endocervical canal. Note 3)
3. Gently rotate the swab twice toobtain cervical mucus. Note 4)

Points to note

1. Collect specimen from the endocervical canal.
2. Collect specimen before washing the vagina.
3. Insert the swab while avoiding contact with anything, such as secretion or mucus around the external os, till it reaches the endocervical canal.
4. Each rotation should take about 5 seconds to allow the swab to absorb mucus. If a small amount of solid matters are attached to the swab, remove them with tweezers.
   
5. Start extraction procedures of the collected specimen within 15 minutes.

[Note]
Please note that the swab shaft may be broken if an excessive force is applied to the shaft while collecting specimen.

Extraction methods

1. Unscrew the blue cap of the extraction container with the extraction buffer.
   
2. Place the cotton swab with the collected cervical mucus in the container, and allow to stand for 2 to 3 minutes.
   Then, shake the swab in an up-and-down direction for 20 to 30 times to extract the specimen.
   
3. Squeeze the cotton swab by each container with fingers to release the specimen extract and remove the swab.
   
4. Set the filter.
   
5. Filter the specimen extract into the specimen storage container. Apply only one pressure to the extraction container to obtain 5 to 7 drops (about 300 μL) of the extract.
   If the required amount of extract is not obtained, start the procedure all over again from specimen collection.
   Seal the specimen storage container with the white cap, and store it. Specimens should be tested within 3 days when refrigerated (8°C) and within 3 months when frozen (≤‒15°C).