TEST DIRECTORY

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Laboratory：Akiruno

○Methotrexate

CODE：00Y65 5(旧 0Y65 9)

  • TEST NAME SPECIMEN
    REQUIREMENT
    （ｍL）     CONTAINER CAP COLOR STORE
    TEMPERATURE
    (STABILITY)     TURNAROUND
    TIME (DAY)     METHODOLOGY REFERENCE RANGE
    (UNIT)     BLOOD COLLECTION TIME
  • Emergency
    Methotrexate
    Serum
    0.7
    S7P
    ↓
    A00     Refrigeration
    （35 days）
     2-4 CLEIA

    CLEIA (Chemiluminescent enzyme immunoassay)
    Chemiluminescent enzyme immunoassay
    After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

     Hazardous limit concentration 24-hour value 10 or more
    48-hour value 1 or more
    72-hour value 0.1 or more (μmol/L)     24, 48, 72 hours during methotrexate/leucovorin rescue therapy
other material

COMMENT


Methotrexate

●Points to note concerning blood collecting containers
Avoid using a container with a serum separating agent. It may effect the results on the drug tests.

●Points to note concerning the blood collecting time
1. The blood collecting time is for TDM (therapeutic drug monitoring).
2. In repetitive administration, collect a blood sample after a steady state is achieved.
3. When poisoning is developed, the peak concentration also needs to be measured.
4. The blood collecting time shows the standard time: a patient’s condition should be taken into account in collecting a blood sample.

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