TEST DIRECTORY

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Laboratory：Akiruno

○POLE gene analysis (uterine body cancer)

CODE：00Y14 1(旧 0Y14 4)

  • TEST NAME SPECIMEN
    REQUIREMENT
    （ｍL）     CONTAINER CAP COLOR STORE
    TEMPERATURE
    (STABILITY)     TURNAROUND
    TIME (DAY)     METHODOLOGY REFERENCE RANGE
    (UNIT)
  • POLE gene analysis (uterine body cancer)
    Unstained specimen slide
    5 sheets
    thickness 5μm
    Z10 Room temperature
     10-14 Direct sequence method

    Direct sequencing method
    A method of directly determining the base sequence using DNA amplified by PCR method as a template.

COMMENT


This test analyzes 11 variants in the POLE gene from pathological specimens, specifically: P286R, M295R, S297F, F367S, D368Y, V411L, L424I, P436R, M444K, A456P, and S459F. We cannot accept requests of anything other than pathological materials.
A tumor cell content of 40% or more is required for this test. For specimens with less than 40% tumor cells, the results may be undeterminable or lead to false-negative outcomes.
Please refer to the notes below regarding the submission of unstained specimen slides.
Please avoid duplicate requests with other items.

●Submission conditions
For unstained specimen slides, please ensure that histopathological evaluation has been performed and that the tumor cell content (percentage of tumor cells among all cells on the slide) is equal to or greater than the required amount for testing. If the tumor cell content is below the required percentage, please mark the tumor cell area on the back of the unstained specimen slide. Please note that if the slide is submitted without marking, macrodissection cannot be performed, which may affect the test results, including the possibility of false-negative findings.

●About the unstained slides
Please immediately fix the collected tissue by immersing it in 10% neutral buffered formalin, with a recommended fixation time of 6 to 48 hours. When submitting, please prepare consecutive sections of the specified thickness from formalin-fixed paraffin-embedded (FFPE) blocks made within the past three years, whenever possible. Please take sufficient care to prevent contamination during sectioning, such as changing the microtome blade for each specimen. Please note that nucleic acids are fragmented due to formalin fixation of the tissue, and depending on the type and composition of the fixative, fixation time, and storage conditions after fixation, analysis may not be possible.

●About biopsy specimens
Please note that biopsy specimens often have only small amount of tissue, and there is a possibility that the tissue has been mostly depleted or that the fragments do not contain tumor cells.

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