TEST DIRECTORY

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Laboratory：Akiruno

○Thyroid Cancer Oncomine DxTT Multi-2 Gene CDxFFPE

CODE：00W53 0

  • TEST NAME SPECIMEN
    REQUIREMENT
    （ｍL）     CONTAINER CAP COLOR STORE
    TEMPERATURE
    (STABILITY)     TURNAROUND
    TIME (DAY)     METHODOLOGY REFERENCE RANGE
    (UNIT)
  • Thyroid Cancer Oncomine DxTT Multi-2 Gene CDxFFPE
    Unstained specimen slide
    5 to 10 sheets
    thickness 5μm
    Z10 Room temperature
     6-11 Next Generation Sequencing (NGS)

    A method that uses a next-generation sequencer to simultaneously determine the base sequences of a huge number of DNA fragments.

COMMENT


The purpose of this test is to analyze RET fusion gene, RET gene mutation, BRAF gene V600E mutation in genomic DNA and RNA extracted from cancer tissue, and to assist in determining the indications for the drugs listed separately for thyroid cancer and medullary thyroid cancer..
The percentage of tumor cells required for the test is at least 30%.
See below for notes on the submission of unstained specimen slides.
Avoid duplicate requests in one sample. Since contamination has a greater effect on this test method, handle specimens with care when collecting them.
When requesting this test, ensure to also request the nucleic acid extraction item (item code: M9514)
●About submission of unstained specimen slides
Immediately immerse the collected tissue in a 10% neutral buffered formalin solution and fix it (recommended fixation time is 6 to 48 hours). For submission, please prepare 5-μm-thick serial sections from formalin-fixed, paraffin-embedded tissue blocks.For submission, please prepare 5-μm-thick serial sections from formalin-fixed, paraffin-embedded tissue blocks. Be careful to avoid contamination by changing the microtome blade for each specimen when slicing. Note that as nucleic acids are fragmented due to formalin fixation of tissues, analysis may not be possible depending on the type and composition of the fixative, fixation time, and storage condition of the specimen after fixation.

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