Laboratory:Akiruno
- TOP
- gene-related test
- genetic tests for malignant tumor (solid tumor)
- AmoyDx Lung Cancer Multipanel 7 Genes for Research (12~24 months)
Laboratory:Akiruno
○AmoyDx Lung Cancer Multipanel 7 Genes for Research (12~24 months)
CODE:00P45 6
-
TEST NAME
SPECIMEN
REQUIREMENT
(mL) CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT) -
AmoyDx Lung Cancer Multipanel 7 Genes for Research (12~24 months)
Unstained specimen slide
7-10 pieces 5μm
Z10
4-7 PCR (Real-time PCR, RT-PCR (Real-time PCR)
COMMENT
The purpose of this study is to analyze BRAF V600E mutations, EGFR gene mutation, ALK fusion gene, ROS1 fusion gene, MET gene exon 14 skipping mutation, KRAS G12C gene mutation, and RET fusion gene in genomic DNA and RNA extracted from cancer tissues to determine the indications for the drugs listed in the attached table for patients with non-small cell lung cancer.
The percentage of tumor cells required for testing is 20% or more (recommended 30% or more). Please see below for notes on submitting unstained specimen slides.
Please avoid duplicate requests with other items. Since this test method is more susceptible to contamination, please handle the specimens with care when collecting them.
When requesting this test, please be sure to also request the nucleic acid extraction item (item code No.: M951 4).
●About submission of Unstained Specimen Slides
・The collected tissue should be immediately immersed in a 10% neutral buffered formalin solution for fixation (Recommended immobilization time is 6-48 hours). When submitting, please prepare serial sections of the 5μm thickness from formalin-fixed, paraffin-embedded (FFPE) blocks. If the tumor cell percentage is less than 20%, mark the tumor area from the back of the unstained specimen slide. Please be aware that if the specimens are submitted without marking, it may affect the results, such as a false negative result. In addition, marked unstained specimen slides should be stored at room temperature and submitted in an object case (Z10).
・Please submit FFPE tissue created within the past 12-24 months.
・Unstained specimen slides may not be analyzed depending on the type and composition of the fixative solution, fixation time, and storage conditions of the specimen after fixation, because nucleic acids are fragmented by formalin fixation of the tissue. Please note that biopsy specimens, in particular, are often minute quantities, and the tissue f
CONTAINER
Z10 旧容器記号 t 30
[オブジェクトケース]
プレパラート (スライドグラス)
貯蔵方法:室温
supplementary information
対象遺伝子変異等と関連する医薬品および適応がん腫
遺伝子変異等 | がん腫 | 関連する医薬品 |
---|---|---|
BRAF V600E変異 | 非小細胞肺癌 | ダブラフェニブメシル酸塩及びトラメチニブジメチルスルホキシド付加物の併用投与 |
EGFR遺伝子変異 | ゲフィチニブ、エルロチニブ塩酸塩、アファチニブマレイン酸塩、オシメルチニブメシル酸塩 | |
ALK融合遺伝子 | クリゾチニブ、アレクチニブ塩酸塩、ブリグチニブ | |
ROS1融合遺伝子 | クリゾチニブ、エヌトレクチニブ | |
MET ex14スキッピング変異 | テポチニブ塩酸塩水和物 | |
KRAS G12C遺伝子変異 | ソトラシブ | |
RET融合遺伝子 | セルペルカチニブ |