TEST DIRECTORY

Table of Test Materials

• Tumor area (1 type from the following materials)
  SPECIMEN REQUIREMENT (mL)	CONTAINER	STORE TEMPERATURE (STABILITY)
  Blood 5.0×2 bottle	PN5	(1 day)
  Bone marrow fluid 1.0×2 bottle	H00	(1 day)
  Unstained specimen slide tissue volume 2mm3 or more	Z10	(3 months after thinning)
  Fresh specimen (body cavity fluid) 10.0 or more	ARR

• Normal area (1 type from the following materials)
  SPECIMEN REQUIREMENT (mL)	CONTAINER	STORE TEMPERATURE (STABILITY)
  Oral mucosa 2 bottles	V92 ↓ ARR	(21 days)
  Nail 20mg or more	ARR	(7 days)

About Oral Mucosa

• Be careful to avoid contamination of leukocytes in blood and saliva.
• After eating, drinking, or breastfeeding, wait 30 minutes before collecting specimens.
• Rinse the mouth thoroughly with water to remove food or other contaminants before collection.
• Use two cottonswabs to rub the side of your right and left inner cheek about 10-15 times each.
• After collection, let the cotton swabs air-dry for about an hour, then place the tip of the swabs in the dedicated container.
• In case customers wish to submit cotton swabs other than the dedicated cotton swabs, please contact us in advance.

About Nails

• Before collection, thoroughly wash the space between the nails and fingers to remove as much dirt and debris as possible.
• To prevent contamination of non-patient's nails, use nail clippers exclusively for the patient.
• Nail polish should be wiped off with nail polish remover before collection.
• Collect the white part of the nail tip of each finger.

Blood and bone marrow fluid

• Collect specimens in the containers shown below, mix well, and store refrigerated. The specimens should be submitted on the same day they are collected.

About Unstained Specimen Slides

• The collected tissue should be immediately immersed in 10% neutral buffered formalin solution and fixed (recommended time: 6-48 hours). When submitting the tissue, prepare 5-μm-thick serial sections from formalin-fixed, paraffin-embedded tissue blocks.
• Unstained specimen slides should be histopathologically evaluated to ensure the presence of at least the required tumor cell percentage (% tumor cells to total cells in the specimen) for the test.
  If the sample does not contain the required percentage of tumor cells, mark the tumor area from the reverse side; otherwise, macrodissection cannot be performed, which may result in false negative or other negative results. Unstained specimen slides should be submitted in an object case (Z10) and stored at room temperature.
• Unstained specimen slides may be impossible to analyze depending on the type and composition of the fixative solution, fixation time, and storage conditions of the specimen after fixation, because nucleic acids are fragmented by formalin fixation of the tissue. As far as possible, specimens collected within the past 3 years should be submitted. Note that biopsy specimens are often minute in quantity, and the tissue fragments themselves on paraffin sections may be small or may not contain tumor cells.
• In order to obtain sufficient nucleic acid content, prepare and submit unstained specimen slides with a total tissue volume of at least 2mm³ (e.g., at least 20 slides for a section thickness of 5µm and a specimen area of 20mm²).

About body cavity fluids

• The collected pleural fluid, alveolar lavage fluid, and other body cavity fluid should be placed in a dedicated container (ARR), centrifuged (760G [2000-3000 rpm] × 10 minutes) at room temperature, and the supernatant should be removed as much as possible.