TEST DIRECTORY

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Laboratory:Akiruno

Oncomine DxTT Multi CDx (Thyroid Cancer) FFPE(Suspended beyond orders placed 06-14-2024)

  • TEST NAME SPECIMEN
    REQUIREMENT
    (mL)
    CONTAINER CAP COLOR STORE
    TEMPERATURE
    (STABILITY)
    TURNAROUND
    TIME (DAY)
    METHODOLOGY REFERENCE RANGE
    (UNIT)
  • INACTIVE
    Oncomine DxTT Multi CDx (Thyroid Cancer) FFPE(Suspended beyond orders placed 06-14-2024)
    Unstained specimen slide
    5 to 10 sheets
    thickness 5?m
    Z10 Room temperature
    6-11 Next Generation Sequencing (NGS)

    A method that uses a next-generation sequencer to simultaneously determine the base sequences of a huge number of DNA fragments.

COMMENT


The purpose of this study is to analyze RET gene mutations and RET fusion genes in genomic DNA and RNA extracted from cancer tissues to determine the indications for the drugs listed in the attached table for thyroid cancer and medullary thyroid carcinoma.
The percentage of tumor cells required for testing is 30% or more. Please see below for notes on submitting unstained specimen slides.
Please avoid duplicate requests with other items. Since this test method is more susceptible to contamination, please handle the specimens with care when collecting them.
When requesting this test, please be sure to also request the nucleic acid extraction item (item code No.: M951 4).
●About submission conditions
Unstained specimen slides should be histopathologically evaluated to ensure that tumor cells are present in a proportion greater than that required for testing. If the tumor cell percentage is not met, mark the tumor cell area from the back of the unstained specimen slide. Please be aware that if the specimens are submitted without   marking, macrodissection cannot be performed, which may affect the results, such as a false negative result.
●About unstained specimen slides
The collected tissue should be immediately immersed in a 10% neutral buffered formalin solution for fixation (Recommended immobilization time is 6-48 hours). When submitting, please prepare serial sections of the specified thickness from formalin-fixed, paraffin-embedded (FFPE) blocks made within the last three years if possible. Please be careful to avoid contamination by changing the microtome blade for each specimen during thin sectioning. Additionally, Please note that since nucleic acids are fragmented by formalin fixation of tissues, analysis may not be possible depending on the type and composition of the fixation solution, fixation time, and storage conditions of the specimens after fixation.
●About biopsy specimens
Please note that biopsy specimens are often minute amounts of specimen, and the tissue itself may be almost lost or may be a piece of tissue that contains no tumor cells.
Please submit at least 10 slides. Specimens with a low number of nucleated cells may not be tested because the required amount of nucleic acid cannot be obtained.
If the biopsy specimen is small (the area of the tissue section is 4mm2 or less [2mm x 2mm or less]), please submit 15 or more specimens.

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