TEST DIRECTORY

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Laboratory：Akiruno

○KRAS G12C mutation analysis

CODE：00G04 0

  • TEST NAME SPECIMEN
    REQUIREMENT
    （ｍL）     CONTAINER CAP COLOR STORE
    TEMPERATURE
    (STABILITY)     TURNAROUND
    TIME (DAY)     METHODOLOGY REFERENCE RANGE
    (UNIT)
  • KRAS G12C mutation analysis
    Unstained specimen slide
    5 sheets
    thickness 5μm
    Z10 Room temperature
     4-10 PCR (Real Time PCR)

    Real-time PCR
    A type of nucleic acid amplification method based on the basic principle of PCR, which uses oligonucleotides that emit fluorescence upon decomposition to quantify target nucleic acids in real time by checking the fluorescent signal at each PCR cycle. A measurement method that enables

COMMENT


The purpose of this test is to analyze KRAS G12C mutations in genomic DNA extracted from cancer tissue to determine the indication of Sotrasib for non-small cell lung cancer.
The recommended percentage of tumor cells for testing is 20% or more.
Avoid duplicate requests in one sample.
Since contamination has a greater effect on this test method, handle specimens with care when collecting them.
●Submission Conditions
Unstained specimen slides should be histopathologically evaluated to ensure that the required percentage of tumor cells (tumor cells as a percentage of total cells in the specimen) is present. If the required percentage is not met, mark the tumor cell area from the back of the unstained specimen slide.  Note that submission of unstained specimen slides without marking may result in failure of macrodissection, which may affect the results, such as a false negative result.
●About unstained specimen slides
Immediately immerse the collected tissue in a 10% neutral buffered formalin solution and fix it (recommended fixation time is 6 to 48 hours). When submitting, please prepare serial sections at the specified thickness from formalin-fixed paraffin-embedded (FFPE) blocks prepared within the last 3 years, if possible. Please be careful to avoid contamination by changing the microtome blade for each specimen when slicing. Please note that as nucleic acids are fragmented due to formalin fixation of tissues, analysis may not be possible depending on the type and composition of the fixative, fixation time, and storage condition of the specimen after fixation.
●About biopsy specimens
Biopsy specimens often contain only a small amount of specimen, and there is a possibility that most of the tissue itself has disappeared or that the tissue has become a piece of tissue that does not contain tumor cells. Be aware of this in advance.

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