Laboratory：Akiruno
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- gene-related test
- genetic tests for hematopoietic tumor
- EZH2 gene mutation analysis
Laboratory：Akiruno
○EZH2 gene mutation analysis
CODE：00D03 4
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TEST NAME
SPECIMEN
REQUIREMENT
（ｍL） CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT) -
EZH2 gene mutation analysis
Unstained specimen slide
5 sheets
thickness 5μm
Z10
7-11 PCR (Real Time PCR)Real-time PCR
A type of nucleic acid amplification method based on the basic principle of PCR, which uses oligonucleotides that emit fluorescence upon decomposition to quantify target nucleic acids in real time by checking the fluorescent signal at each PCR cycle. A measurement method that enables
COMMENT
The purpose is to analyze mutations in the EZH2 gene (Y646N, Y646F, Y646X, A682G, A692V) in pathology material.The purpose is to analyze mutations in the EZH2 gene (Y646N, Y646F, Y646X, A682G, A692V) in pathology material. The percentage of tumor cells required for the test is 15% or more.
Please see the below for the notes on submitting unstained specimen slides.
Avoid duplicate requests for other items.
● Submission Conditions
Unstained specimen slides should be histopathologically evaluated to ensure that the required percentage of tumor cells (tumor cells as a percentage of total cells in the specimen) is present. If the required percentage is not met, mark the tumor cell area from the back of the unstained specimen slide. Note that submission of unstained specimen slides without marking may result in failure of macrodissection, which may affect the results, such as a false negative result. ●About unstained specimen slides
Immediately immerse the collected tissue in a 10% neutral buffered formalin solution and fix it (recommended fixation time is 6 to 48 hours). When submitting, please prepare serial sections at the specified thickness from formalin-fixed paraffin-embedded (FFPE) blocks prepared within the last 3 years, if possible. Please be careful to avoid contamination by changing the microtome blade for each specimen when slicing. Please note that as nucleic acids are fragmented due to formalin fixation of tissues, analysis may not be possible depending on the type and composition of the fixative, fixation time, and storage condition of the specimen after fixation.
CONTAINER
![[オブジェクトケース] プレパラート (スライドグラス)](../../common/yoki-images/z10.jpg)
Z10 旧容器記号 t 30
[オブジェクトケース]
プレパラート (スライドグラス)
貯蔵方法：室温
