Pregnenolone（Suspended beyond orders placed 12-05-2019）

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endocrinology test
adrenocortical hormones and their binding protein
Pregnenolone（Suspended beyond orders placed 12-05-2019）

Laboratory：Akiruno

○Pregnenolone（Suspended beyond orders placed 12-05-2019）

CODE：00963 2

TEST NAME SPECIMEN
REQUIREMENT
（ｍL） CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT)
Pregnenolone（Suspended beyond orders placed 12-05-2019）
Serum
0.9
S09
↓
A00
8-21 RIA ammonium sulfate salting out method
RIA (Radio immunoassay)
Radioimmunoassay
A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity.
As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.
M0.10-1.00
F0.20-1.50
(ng/mL)

TEST NAME	SPECIMEN REQUIREMENT （ｍL）	CONTAINER
Pregnenolone（Suspended beyond orders placed 12-05-2019）	Serum 0.9	S09 ↓ A00

CAP COLOR	STORE TEMPERATURE (STABILITY)	TURNAROUND TIME (DAY)
		8-21

METHODOLOGY	REFERENCE RANGE (UNIT)
RIA ammonium sulfate salting out method RIA (Radio immunoassay) Radioimmunoassay A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity. As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.	M0.10-1.00 F0.20-1.50 (ng/mL)

METHODOLOGY

REFERENCE RANGE
(UNIT)

RIA ammonium sulfate salting out method

RIA (Radio immunoassay)
Radioimmunoassay
A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity.
As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.

M0.10-1.00
F0.20-1.50
(ng/mL)

CONTAINER

S09

With separating agent (9mL vacuum blood sampling tube)

Storage method: Room temperature
Expiration period: 1 year from production

A00 Previous container symbol X

Polyethylene test tube

Storage method: Room temperature

TEST DIRECTORY