Total estrogen for non-pregnant women（Suspended beyond orders placed 12-05-2019）

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endocrinology test
gonadal hormones, placental hormones and their binding protein
Total estrogen for non-pregnant women（Suspended beyond orders placed 12-05-2019）

Laboratory：Akiruno

○Total estrogen for non-pregnant women（Suspended beyond orders placed 12-05-2019）

CODE：00813 8

TEST NAME SPECIMEN
REQUIREMENT
（ｍL） CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT)
Total estrogen for non-pregnant women（Suspended beyond orders placed 12-05-2019）
Urine storage
5.0
U00
7-18 RIA ammonium sulfate salting out method
RIA (Radio immunoassay)
Radioimmunoassay
A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity.
As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.
See below

TEST NAME	SPECIMEN REQUIREMENT （ｍL）	CONTAINER
Total estrogen for non-pregnant women（Suspended beyond orders placed 12-05-2019）	Urine storage 5.0	U00

CAP COLOR	STORE TEMPERATURE (STABILITY)	TURNAROUND TIME (DAY)
		7-18

METHODOLOGY	REFERENCE RANGE (UNIT)
RIA ammonium sulfate salting out method RIA (Radio immunoassay) Radioimmunoassay A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity. As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.	See below

METHODOLOGY

REFERENCE RANGE
(UNIT)

RIA ammonium sulfate salting out method

RIA (Radio immunoassay)
Radioimmunoassay
A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity.
As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.

See below

COMMENT

Please keep urine for 24 hours in a cool, dark place, mix well, and submit the required amount. Please enter your gender (sexual cycle).

CONTAINER

U00 Previous container symbol Y

Urine containers

Storage method: Room temperature

supplementary information

(単位：μg/day)
女性	卵胞期	3.00～20.0
排卵期	10.0～60.0
黄体期	8.00～50.0
閉経後	10.0以下
男性	2.00～20.0

Reference ranges of estrogen total, Non-pregnant woman

(Unit：μg/day)
Female	Follicular phase	3.00 – 20.0
	Ovulatory phase	10.0 – 60.0
	Luteal phase	8.00 – 50.0
	Postmenopausal	≤ 10.0
Male		2.00 – 20.0

TEST DIRECTORY