TEST DIRECTORY

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Laboratory：Akiruno

○Culture identification

CODE：00666 2

  • TEST NAME TESTING MATERIAL CONTAINER CAP COLOR STORE
    TEMPERATURE
    (STABILITY)     METHODOLOGY TURNAROUND
    TIME (DAY)
  • Culture identification
    Specimens from the oral cavity, respiratory tract, or respiratory organs (sputum, throat swab fluid)
    See below Refrigeration
     Culture identification and MALDI-TOF-MS 3-6

TEST NAME

TESTING MATERIAL

CONTAINER

Culture identification

Specimens from the oral cavity, respiratory tract, or respiratory organs (sputum, throat swab fluid)

CAP COLOR

STORE
TEMPERATURE
(STABILITY)

METHODOLOGY

Refrigeration

Culture identification and MALDI-TOF-MS

TURNAROUND
TIME (DAY)

3-6

COMMENT


For bacterial culture identification tests, an emergency reporting range (see below) has been set, and if the range is exceeded, we will issue an emergency report.
Note) Depending on the details of the requested inspection, it may take more days.

supplementary information

Result interpretation criteria for common bacteria

Test results for items listed below will be reported in the following manner, according to the following SRL criteria:
(Reference: Clinical Microbiology Procedures Handbook - 4th edition)

TEST NAME Category and
microscope magnification power		 Result displayed Bacterial count/cell count Comment
Microscopic
examination of
smears		 Bacterial count※
(1000×
magnification)		 (－) No bacteria detected
1＋ 1 field < 1
2＋ 1 field 1-5
3＋ 1 field 6-30
4＋ 1 field > 30
Cell count (WBC/epithelial cells)
(100×
magnification)		 (－) No cell detected
1＋ 1 field < 1
2＋ 1 field 1-9
3＋ 1 field 10-25
4＋ 1 field > 25

※ In the case of a culture bottle and a strain, the indication method of the number of bacteria becomes "positive".

TEST NAME Result displayed Growth in medium Comment
Culture and
identification		 (－) No growth The species of bacteria are reported according to the International Code of Nomenclature of Bacteria.
1＋ < 1/3
2＋ ≥ 1/3 to < 2/3
3＋ ≥ 2/3
4＋ Entire surface of the medium
TEST NAME Result displayed Susceptibility Comment
Drug
susceptibility
testing		 S Susceptible Results are reported according to the CLSI criteria.
I Intermediate
R Resistant
CLSI: Clinical and Laboratory Standards Institute (former NCCLS)

Criteria for harvesting bacterial colonies in major samples

The process of collecting bacteria for the identification of common bacteria by culture will be performed according to the following SRL criteria:

  • Bacteria Sputum,
    throat    		 Urine Vaginal
    secretion    		 Blood,
    cerebrospinal fluid
    Staphylococcus aureus ○ ○ ○ ◎
    Staphylococcus species,
    coagulase-negative    		 ◆
    Note 1
    		 ○ ○ ◎
    β-hemolytic streptococci ◎ ○ ◎ ◎
    Streptococcus pneumoniae ◎ ○ ○ ◎
    Enterococcus ◆
    Note 1
    		 ○ ○ ◎
    Enterobacteria ◆
    Note 2, Note 3
    		 ○ ○ ◎
    Pseudomonas aeruginosa ○ ○ ○ ◎
    Non-fermentative bacteria ◆
    Note 1
    		 ○ ○ ◎
    Haemophilus ◆
    Note 2
    		 － ○ ◎
    Branhamella ◆
    Note 2
    		 － － ◎
  • Symbols and interpretations

    ◎：Collected if 1 colony is detected
    ○：Collected if 1+ colonies are detected
    ◆：Collected if 2+ colonies are detected
    －：Not collected
    Note 1：Not collected unless there is significant growth
    Note 2：Collected if there is significant growth even with 1+ colonies
    Note 3：Collected in the case of Klebsiella pneumoniae even with 1+ colonies

Other bacteria will be collected only when a target is specifically requested for. (Please see below for the list of target bacteria.)
If specifically requested, a target bacteria will be collected even if there is only 1 colony.

Target bacteria

  • CODE Target bacteria
    		Diarrheagenic Escherichia coli
    (including O157)
    		Escherichia coli O157
    		Yersinia enterocolitica
    		Bordetella pertussis
    		Neisseria gonorrhoeae
    室温
    		Neisseria meningitidis
    室温
    		Campylobacter
    		Legionella
    		Corynebacterium diphtheriae
  • CODE Target bacteria
    		Shigella
    		Salmonella
    		Vibrio parahaemolyticus
    		Vibrio cholerae
    		Pseudomonas aeruginosa
    		Haemophilus
    		Staphylococcus
    		MRSA
    		Streptococcus pneumoniae
  • CODE Target bacteria
    		Group A streptococci
    		Group B streptococci (GBS)
    		Clostridium difficile
    		Clostridium perfringens
    		Candida
    		Cryptococcus
    		Aspergillus
    		Trichophyton
    		Filamentous fungi
Comment (common in all target bacteria)
  • A request only for target bacteria is not accepted. Make sure to also place an order of culture and identification of common bacteria as well.
  • When requesting a test of Clostridium difficile and Clostridium perfringens, make sure to also place an order of anaerobic culture as well. If no target bacterium is stated in a request of anaerobic culture of feces, the target bacterium is considered as Clostridium difficile.
  • When requesting a test of diarrheagenic Escherichia coli or Escherichia coli O157, make sure to also place an order of culture and identification (digestive organs) as well.
    (Note) Please note that if no check mark is placed, the test will not be conducted.
  • MRSA is determined based on a result of drug susceptibility. If no request if made for drug susceptibility testing, the determination is made based on a result from screening medium. For a more reliable determination, we recommend you to also request the drug susceptibility testing as well.
  • For Bordetella pertussis, Legionella, and Corynebacterium diphtheriae, a test is performed only when the corresponding field(s) is checked.
  • Up to 10 bacteria can be chosen as the target bacteria. Even if a request is made with more target bacteria, a report cannot include all.

List of dedicated transport containers and transport media for microbiological tests

Category Specimen Specimen
requirement (mL)		 Storage
conditions		 Collecting container
Oral cavity /
Airway /
Respiratory organs		 Sputum 2 - 3 Refrigeration X00 Sterile sputum collection container
Throat swab As appropriate Refrigeration VS1 Seed-Swab γ-1
Digestive organs Stool As appropriate Refrigeration F01 Stool container with Cary Blair medium
Bile 5 - 10 Refrigeration ARR Sterile tube (10mL)
Gastric juice 2 - 3 Refrigeration ARR Sterile tube (10mL)
Urinary /
Reproductive organs		 Midstream urine 5 - 10 Refrigeration ARR Sterile tube (10mL)
Catheter urine 5 - 10 Refrigeration ARR Sterile tube (10mL)
vaginal secretion As appropriate Refrigeration VS1 Seed-Swab γ-1
Pus As appropriate Refrigeration VS1 Seed-Swab γ-1
Blood / Aspirate Venous blood Optimum
8 - 10		 Room temperature CBG
CBS		 Blood culture bottle
Arterial blood Room temperature CBG
CBS		 Blood culture bottle
Cerebrospinal
fluid		 2 - 3 Room temperature C10 Anaerobic porter
Pleural fluid 5 - 10 Refrigeration C10 Anaerobic porter
Ascites 5 - 10 Refrigeration C10 Anaerobic porter
synovial fluid 5 - 10 Refrigeration C10 Anaerobic porter
Other sites Otorrhoea As appropriate Refrigeration VS2 Seed-Swab γ-2
Eye discharge As appropriate Refrigeration VS1 Seed-Swab γ-1
Skin As appropriate Refrigeration ARR Sterile tube (10mL)
Nail As appropriate Refrigeration ARR Sterile tube (10mL)
tissues As appropriate Refrigeration ARR Sterile tube (10mL)
Pus As appropriate Refrigeration C10 Anaerobic porter
Others Environmental
material		 - Room temperature C30 Medium for stamp method (for hospital environmental test)
- Refrigeration VS1 Seed-Swab γ-1
Gastric biopsy tissues As appropriate Refrigeration C20 Seed Tube HP
(for culture and identifi cation of Helicobacter)
Stool 1.0g Refrigeration F00 Stool container
Sterile polyethylene test tube

ARR　Previous container symbol r

Sterile polyethylene test tube

Storage conditions: Room temperature

C10

C10　Previous container symbol n

C20

C20　Previous container symbol n2

C30

C30　Previous container symbol n3

CBG

CBG　Previous container symbol m1

CBS

CBS　Previous container symbol q1

Stool container

F00　Previous container symbol U

Stool container

Storage conditions: Room temperature

F01

F01　Previous container symbol d3

VS1

VS1　Previous container symbol k

VS2

VS2　Previous container symbol k1

Sterile sputum collection container

X00　Previous container symbol l (Small letter L)

Sterile sputum collection container

Storage conditions: Room temperature

Collecting samples for bacteria testing

  • Make sure that sample is clearly labeled with the name of testing material or the harvest site.
  • If the target bacterium is Neisseria gonorrhoeae or Neisseria meningitidis, Room temperature store them at room temperature.
  • Collect materials for the anaerobic culturing in an anaerobic porter.
  • Please note that the number of days required for testing varies depending on the target bacteria, etc.

Handling an anaerobic porter

(A) For a fluid specimen　(e.g., pleural fluid, ascites, urine, aspirate)
(B) For a solid specimen　(Stool, pus, tissues, etc)

Specimen handling methods for culture and identification of Helicobacter

Handling method of Seed Tube HP

  1. 1. Immediately after obtaining “ gastric biopsy tissues,” place it into a collecting container (C20) under the black line.
  2. 2. Place the sample in the Refrigeration storage within 30 minutes from sampling, and put in your request on the same day.

Collecting samples for environmental test for wiping

Hospital environmental test (wiping method)

Wipe an area (typically 10 × 10 cm) on the analyte with a cotton swab included in Seed Swab γ-1, which has been damped by placing it in a culture medium. Place it back in the culture medium before submitting for testing.

Hospital environmental test (stamping method)

Remove the cap from the stamp medium (C30), and gently press the surface of the medium onto the surface of the analyte, taking care not to touch the medium surface with a finger, etc. After approximately 5 seconds, replace the cap and send it in for testing.

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