B cell surface immune globulin (Sm-Ig) IgA

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cellular immunity test
cellular immunity test
B cell surface immune globulin (Sm-Ig) IgA

Laboratory：Akiruno

○B cell surface immune globulin (Sm-Ig) IgA

CODE：00531 1

TEST NAME SPECIMEN
REQUIREMENT
（ｍL） CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT)
B cell surface immune globulin (Sm-Ig) IgA
Blood (heparin added)
1.0
PH5
3-5 Flow cytometry(FCM)
Flow cytometry
A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen).
Two-color flow cytometry uses a combination of fluorochromes for double staining.
1-3(%)

TEST NAME	SPECIMEN REQUIREMENT （ｍL）	CONTAINER
B cell surface immune globulin (Sm-Ig) IgA	Blood (heparin added) 1.0	PH5

CAP COLOR	STORE TEMPERATURE (STABILITY)	TURNAROUND TIME (DAY)
		3-5

METHODOLOGY	REFERENCE RANGE (UNIT)
Flow cytometry(FCM) Flow cytometry A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen). Two-color flow cytometry uses a combination of fluorochromes for double staining.	1-3(%)

METHODOLOGY

REFERENCE RANGE
(UNIT)

Flow cytometry(FCM)

Flow cytometry
A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen).
Two-color flow cytometry uses a combination of fluorochromes for double staining.

1-3(%)

COMMENT

Acceptable days are Monday to Friday.
About requesting cell-mediated immunity testing
1. Please submit the specimen on the same day after collection.
2. If there are few lymphocytes, please collect more blood.
3. If requesting bone marrow fluid, please use a special container (H00) and submit it refrigerated. However, for CD34 quantitative, if it is diluted with storage solution, correct test results will not be obtained, so please use a special container (PH5).
4. If you are requesting lymph node or other tissue, please suspend it in a special container (H20) and refrigerate it.
Collect blood into the container shown below, mix well, and store at room temperature (17 to 25℃).
If there are not enough lymphocytes, please collect more blood.
(3.0mL if requesting 3 or more items)
Please submit the specimen on the same day after collection.

CONTAINER

PH5 Previous container symbol G

With heparin (5mL vacuum blood sampling tube)

Contents: Heparin sodium 65IU
Storage method: Room temperature
Expiration period: 2 years from production

TEST DIRECTORY