Laboratory：Akiruno
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- Lymphocyte rejuvenation test using PHA
Laboratory：Akiruno
○Lymphocyte rejuvenation test using PHA
CODE：00523 0
-
TEST NAME
SPECIMEN
REQUIREMENT
（ｍL） CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT) -
Lymphocyte rejuvenation test using PHA
Blood (heparin added)
5.0
PH9
7-8 3H-thymidine uptake abilityPHA+ 20500-568003H-thymidine uptake capacity (3H-TdR uptake)
A method using the phenomenon that lymphocytes become blastic in response to stimulation by non-self antigens.
Lymphocytes are cultured with the addition of the stimulants and 3H-thymidine, and the amount of 3H-thymidine incorporated into the cells by DNA synthesis is measured as radioactivity. PHA, ConA, and drugs are used for stimulants.
CONTROL 127-456
(cpm)
COMMENT
Acceptable days are Monday to Friday.
Collect blood into the container shown below, mix well, and store at room temperature (17 to 25℃).
If the number of lymphocytes is low, please collect more blood.
(8.0mL if requesting 2 items at the same time)
Please submit the specimen on the same day after collection.
CONTAINER
PH9 旧容器記号 I
ヘパリン入り (真空採血量10mL)
内容：ヘパリンNa 130IU
貯蔵方法：室温
有効期間：製造から2年
