Drug-induced lymphocyte stimulation test (DLST)

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cellular immunity test
cellular immunity test
Drug-induced lymphocyte stimulation test (DLST)

Laboratory：Akiruno

○Drug-induced lymphocyte stimulation test (DLST)

CODE：00517 7

TEST NAME SPECIMEN
REQUIREMENT
（ｍL） CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT)
Drug-induced lymphocyte stimulation test (DLST)
Blood (heparin added)
1 medical drug 12.0
PH9
8-10 ³H-thymidine uptake ability
³H-thymidine uptake capacity (³H-TdR uptake)
A method using the phenomenon that lymphocytes become blastic in response to stimulation by non-self antigens.
Lymphocytes are cultured with the addition of the stimulants and ³H-thymidine, and the amount of ³H-thymidine incorporated into the cells by DNA synthesis is measured as radioactivity. PHA, ConA, and drugs are used for stimulants.
Negative

COMMENT

Acceptable days are Monday to Friday. 5.0mL of blood is required for each additional drug. Please submit your requested medications along with your blood. (As a general rule, intradermal reaction injections cannot be tested.) If you suspect that heparin is the causative agent, use (PNK) containers for all blood collection tubes.
About specimens for drug-induced lymphocyte stimulation test (DLST)
1.1 For each increase in drug 5.0mL of blood is required.
2. One drug requires 5 million lymphocytes. In some cases, it may not be possible to obtain the required number of lymphocytes for the test, making the test impossible.
3. As a guideline, if your white blood cell count is 3000/μL or less, please draw twice the amount of blood and submit it. If you know the detailed white blood cell count and lymphocyte percentage, contact your sales representative.
4. If you are requesting two or more items, be sure to indicate the order of priority.
5. Please submit the requested medication along with the blood.
Tablet (1 tablet), Capsule (1 capsule), Powder (about 1 package per dose), Liquid type oral medicine (about 0.5mL), Injection (1 vial【ampoule】). Test ampoules for intradermal reactions cannot be tested due to the small amount. If you would like to request testing for other drugs, contact your sales representative.
6. If you suspect that heparin is the causative drug, collect all blood in a special container (PNK) and submit it.
After collecting the specimen, submit it on the same day.
7. We cannot accept controlled drugs as listed in Article 2, Chapter I of the "Narcotics and Psychotropics Control Act" and the Appended Table of the "Stimulants Control Act".
8. When making a request, make sure that no narcotics or stimulants are contained and sign the request form, etc. If you do not sign the form, we cannot accept your request.
After collecting the specimen, submit it on the same day.

CONTAINER

PH9 旧容器記号 I

ヘパリン入り (真空採血量10mL)

内容：ヘパリンNa　130IU
貯蔵方法：室温
有効期間：製造から2年

supplementary information

DLST薬剤リスト

※薬剤リストは2024年7月更新予定です。

TEST DIRECTORY