Laboratory:Akiruno
- TOP
- cellular immunity test
- *Lymphocyte surface marker automate analysis by monoclonal antibodies
- CD22
Laboratory:Akiruno
○CD22
CODE:00494 3
-
TEST NAME
SPECIMEN
REQUIREMENT
(mL) CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT) -
CD22
Blood (heparin added)
3.0
PH5
3-5 Flow cytometry(FCM)2.0-17.0(%)Flow cytometry
A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen).
Two-color flow cytometry uses a combination of fluorochromes for double staining.
COMMENT
Acceptable days are Monday to Friday.
Please see below for the list of implementation items.
If you request 10 or more items, please collect 5.0mL of blood.
About requesting a cell-mediated immunity test
1. Please submit the specimen on the same day after collection.
2. If there are few lymphocytes, please collect more blood.
3. If requesting bone marrow fluid, please use a special container (H00) and submit it refrigerated. However, for CD34 quantitative, if it is diluted with storage solution, correct test results will not be obtained, so please use a special container (PH5).
4. If you are making a request for a tissue such as lymph node, please suspend it in a special container (H20) and submit it in a refrigerator.
Collect blood into the container shown below, mix well, and store at room temperature.
(For 10 or more items, please collect 5.0mL of blood.)
After collecting the specimen, please submit it on the same day.
CONTAINER
PH5 旧容器記号 G 12
ヘパリン入り (真空採血量5mL)
内容:ヘパリンNa 65IU
貯蔵方法:室温
有効期間:製造から2年
supplementary information
モノクローナル抗体によるリンパ球表面マーカーの自動解析実施項目一覧
項目コード 新項目コード |
CD No. JLAC10 |
基準値 (単位) | 実施料 判断料 |
主として反応する細胞 代表的な反応性 |
---|---|---|---|---|
5005 6
3946 8
017504401
352401
3524
|
CD1a
5I060-0000-019-662 |
1.0 以下 (%) | 胸腺皮質細胞 | |
5006 3
3945 9
017514401
352601
3526
|
CD2
5I064-0000-019-662 |
71.0~91.0 (%) | T細胞・Eロゼット形成細胞 | |
5001 7
3972 9
017464401
352801
3528
|
CD3
5I067-0000-019-662 |
58.0~84.0 (%) | 185 ※6 |
成熟 (末梢) T細胞 |
5002 4
3973 8
017474401
353001
3530
|
CD4
5I070-0000-019-662 |
25.0~54.0 (%) | ヘルパーT細胞 | |
5003 1
3974 7
017484401
353601
3536
|
CD8
5I082-0000-019-662 |
23.0~56.0 (%) | 細胞傷害性T細胞 | |
CD4/CD8 比 | 0.40~2.30 | |||
5039 1
3956 7
017594401
353201
3532
|
CD5
5I073-0000-019-662 |
60.0~90.0 (%) | T細胞・一部のB細胞 | |
0011 3
3964 8
010024401
353301
3533
|
CD7
5I079-0000-019-662 |
72.0~90.0 (%) | T細胞 | |
5053 9
3957 6
017674401
353701
3537
|
CD10
5I088-0000-019-662 |
1.0 以下 (%) | ALL・好中球 | |
5011 2
3976 5
017534401
366301
3663
|
CD11b
5I092-0000-019-662 |
18.0~49.0 (%) | NK細胞・T細胞の一部 | |
0021 9
3948 6
010094401
354001
3540
|
CD13
5I098-0000-019-662 |
9.0 以下 (%) | 単球・顆粒球・骨髄細胞 | |
0022 6
3952 1
010104401
354101
3541
|
CD14
5I101-0000-019-662 |
5.0 以下 (%) | 単球 | |
5031 3
3958 5
017584401
354301
3543
|
CD16
5I110-0000-019-662 |
6.0~39.0 (%) | NK細胞 (Fcγレセプター) 好中球 | |
0023 3
3959 4
010114401
354401
3544
|
CD19
5I119-0000-019-662 |
5.0~24.0 (%) | B細胞 | |
5359 3
3947 7
018294401
354501
3545
|
CD20
5I122-0000-019-662 |
3.0~20.0 (%) | 成熟B細胞 | |
0036 1
3966 6
010184401
354601
3546
|
CD21
5I125-0000-019-662 |
3.0~18.0 (%) | 成熟B細胞 | |
0494 3
3898 2
012474401
354701
3547
|
CD22
5I128-0000-019-662 |
2.0~17.0 (%) | B細胞 | |
5360 3
3960 2
018304401
354901
3549
|
CD25
5I137-0000-019-662 |
8.0~34.0 (%) | IL-2レセプター Tac | |
0495 1
3900 8
012484401
355001
3550 |
CD30
5I152-0000-019-662 |
1.0 以下 (%) | 活性化T・B細胞・ホジキン細胞 | |
0169 2
3967 5
011074401
355101
3551
|
CD33
5I161-0000-019-662 |
5.0 以下 (%) | 顆粒球・単球・骨髄細胞 | |
0497 5
3899 1
012494401
355201
3552
|
CD34
5I164-0000-019-662 |
1.0 以下 (%) | 造血幹細胞 | |
5008 8
3977 4
017524401
355501
3555
|
CD38
5I176-0000-019-662 |
18.0~59.0 (%) | 活性化および増殖細胞 形質細胞 (強陽性) | |
0184 7
3968 4
011204401
355601
3556
|
CD56
5I238-0000-019-662 |
9.0~43.0 (%) | NK細胞 | |
5030 6
3961 1
017574401
355701
3557
|
CD57
5I241-0000-019-662 |
4.0~45.0 (%) | NK細胞・LGL | |
5004 9
3979 2
017494401
364301
3643
|
HLA-DR
5I680-0000-019-662 |
11.0~46.0 (%) | B細胞・活性化T細胞 (HLA-DR抗原) 単球 |