Amino acid analysis(41 types)

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biochemical test
low molecular nitrogen compounds
Amino acid analysis(41 types)

Laboratory：Akiruno

○Amino acid analysis(41 types)

CODE：00135 1

TEST NAME SPECIMEN
REQUIREMENT
（ｍL） CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT)
Amino acid analysis(41 types)
acidic urine storage
2
U00
（21 days）
7-10 HPLC
HPLC (High performance liquid chromatography)
High performance liquid chromatography
A method of liquid chromatography that uses a liquid as a mobile phase to achieve high speed and high precision separation using a densely packed column and a high pressure pump.
See below

COMMENT

￡
Collect urine in a bottle containing about 20 mL of 6N hydrochloric acid for 24 hours. After mixing it well, submit the necessary amount under frozen conditions.
If the amount of urine is small, put 1 mL of 6N hydrochloric acid per 100 mL of urine.
Be sure to maintain acid urine (pH1.0-3.0).

CONTAINER

U00 旧容器記号 Y

尿用容器

貯蔵方法：室温

supplementary information

[0951, 0089, 0954] アミノ酸分析 (39種類 (血漿)、41種類 (尿)、9種類 (血漿)) 基準値

単位　血漿：nmol/mL、尿：μmol/day (ただし◎印 (Urea) はmmol/day)
アミノ酸名	略号	基準値
アミノ酸名	略号	血漿	尿
Taurine	Tau	39.5～93.2	322.2～5214.5
Phosphoethanolamine★	PEA		31.0～110.0
Urea★	Urea		◎130.3～493.2
Aspartic acid	Asp	2.4以下	12.7以下
Hydroxyproline	Hypro	21.6以下	ND
Threonine	Thr	66.5～188.9	79.9～528.3
Serine	Ser	72.4～164.5	208.8～1020.0
Asparagine	Asn	44.7～96.8	60.7～372.3
Glutamic acid	Glu	12.6～62.5	11.3～42.7
Glutamine	Gln	422.1～703.8	207.0～1357.3
Sarcosine	Sarco	TR	99.0以下
α-Aminoadipic acid	α-AAA	ND	16.7～118.6
Proline	Pro	77.8～272.7	ND
Glycine	Gly	151.0～351.0	652.1～3670.6
Alanine	Ala	208.7～522.7	141.2～833.9
Citrulline	Cit	17.1～42.6	13.5～55.6
α-Aminobutyric acid	α-ABA	7.9～26.6	27.1以下
※Valine	Val	147.8～307.0	24.8～82.2
Cystine	Cys	13.7～28.3	23.7～170.9
Cystathionine	Cysthio	TR	TR～44.7
※Methionine	Met	18.9～40.5	TR～20.2
※Isoleucine	Ileu	43.0～112.8	7.5～23.5
※Leucine	Leu	76.6～171.3	24.6～89.3
※Tyrosine	Tyr	40.4～90.3	50.6～308.4
※Phenylalanine	Phe	42.6～75.7	27.2～110.2
γ-Amino β-hydroxy butyric acid	γ-A β-HBA	ND	ND
β-Alanine	β-Ala	TR	TR～153.0
β-Amino-iso-butyric acid	β-AIBA	TR	TR～1623.9
γ-Aminobutyric acid	γ-ABA	ND	ND
Monoethanolamine★	MEA	10.4以下	195.3～606.2
Homocystine	Homocys	ND	ND
※Histidine	His	59.0～92.0	436.4～2786.5
3-Methylhistidine	3-Me His	5.0以下	113.4～480.9
1-Methylhistidine	1-Me His	18.5以下	59.3～2816.2
Carnosine★	Carno	ND	87.6以下
Anserine★	Ans	ND	231.4以下
Tryptophan	Trp	37.0～74.9	20.7～150.7
Hydroxylysine	Hylys	ND	22.9以下
Ornithine	Orni	31.3～104.7	6.9～43.9
※Lysine	Lys	108.7～242.2	51.6～1639.6
※Arginine	Arg	53.6～133.6	11.6～54.8
Total AA (総アミノ酸：非必須アミノ酸の和+必須アミノ酸の和)		2068.2～3510.3
NEAA (非必須アミノ酸の和)		1381.6～2379.4
EAA (必須アミノ酸の和)		660.0～1222.3
BCAA (分岐鎖アミノ酸の和)		265.8～579.1
EAA/NEAA (必須アミノ酸の和/非必須アミノ酸の和)		0.40～0.63
BCAA/Total AA (分岐鎖アミノ酸の和/総アミノ酸)		0.11～0.18
フィッシャー比		2.43～4.40

※印はアミノ酸分析 (9種類) の実施アミノ酸名
ND：測定感度以下　TR：痕跡
★印は「アミノ酸」ではありませんが、本項目に含めて測定いたします。
EAA/NEAA、BCAA/Total AA、フィッシャー比においては単位なし

Reference Ranges for Amino Acid Analysis (39 Types [Plasma], 41 Types [Urine], 9 Types [Plasma])

Unit: nmol/mL (Plasma), μmol/day (Urine) (however, urea [indicated by "2)"] is mmol/day.)
Amino Acid Name	Abbreviation	Reference Range
Amino Acid Name	Abbreviation	Plasma	Urine
Taurine	Tau	39.5-93.2	322.2-5214.5
Phosphoethanolamine 1)	PEA		31.0-110.0
Urea 1)	Urea		2) 130.3-493.2
Aspartic acid	Asp	2.4 or less	12.7 or less
Hydroxyproline	Hypro	21.6 or less	ND
Threonine	Thr	66.5-188.9	79.9-528.3
Serine	Ser	72.4-164.5	208.8-1020.0
Asparagine	Asn	44.7-96.8	60.7-372.3
Glutamic acid	Glu	12.6-62.5	11.3-42.7
Glutamine	Gln	422.1-703.8	207.0-1357.3
Sarcosine	Sarco	TR	99.0 or less
Alpha-Aminoadipic acid	Alpha-AAA	ND	16.7-118.6
Proline	Pro	77.8-272.7	ND
Glycine	Gly	151.0-351.0	652.1-3670.6
Alanine	Ala	208.7-522.7	141.2-833.9
Citrulline	Cit	17.1-42.6	13.5-55.6
Alpha-Aminobutyric acid	Alpha-ABA	7.9-26.6	27.1 or less
* Valine	Val	147.8-307.0	24.8-82.2
Cystine	Cys	13.7-28.3	23.7-170.9
Cystathionine	Cysthio	TR	TR-44.7
* Methionine	Met	18.9-40.5	TR-20.2
* Isoleucine	Ileu	43.0-112.8	7.5-23.5
* Leucine	Leu	76.6-171.3	24.6-89.3
* Tyrosine	Tyr	40.4-90.3	50.6-308.4
* Phenylalanine	Phe	42.6-75.7	27.2-110.2
Gamma-Amino-beta-hydroxybutyric acid	Gamma-A Beta-HBA	ND	ND
Beta-Alanine	Beta-Ala	TR	TR-153.0
Beta-Aminoisobutyric acid	Beta-AIBA	TR	TR-1623.9
Gamma-Aminobutyric acid	Gamma-ABA	ND	ND
Monoethanolamine 1)	MEA	10.4 or less	195.3-606.2
Homocystine	Homocys	ND	ND
* Histidine	His	59.0-92.0	436.4-2786.5
3-Methylhistidine	3-Me His	5.0 or less	113.4-480.9
1-Methylhistidine	1-Me His	18.5 or less	59.3-2816.2
Carnosine 1)	Carno	ND	87.6 or less
Anserine 1)	Ans	ND	231.4 or less
Tryptophan	Trp	37.0-74.9	20.7-150.7
Hydroxylysine	Hylys	ND	22.9 or less
Ornithine	Orni	31.3-104.7	6.9-43.9
* Lysine	Lys	108.7-242.2	51.6-1639.6
* Arginine	Arg	53.6-133.6	11.6-54.8
Total AA (Total amino acid: Sum of non-essential amino acids + Sum of essential amino acids)		2068.2-3510.3
NEAA (Sum of non-essential amino acids)		1381.6-2379.4
EAA (Sum of essential amino acids)		660.0-1222.3
BCAA (Sum of branched-chain amino acids)		265.8-579.1
EAA/NEAA (Sum of essential amino acids/Sum of non-essential amino acids)		0.40-0.63
BCAA/Total AA (Sum of branched-chain amino acids/Total amino acid)		0.11-0.18
Fischer's ratio		2.43-4.40

Asterisks (*) indicate the amino acid names subjected to the amino acid analysis (9 types).
ND: Below the sensitivity of detection
TR: Trace
Although the substances indicated by "1)" are not "amino acids", we will include and measure them in this item.
No units for EAA/NEAA, BCAA/Total AA, and Fischer's ratio.

TEST DIRECTORY